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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   KV1.7 Antibody   

KV1.7 Antibody

Affinity purified polyclonal antibody

  • WB - KV1.7 Antibody AG1113-050
    Western blot analysis of mouse heart membranes:
    1. Anti-KV1.7 antibody (#AG1113), (1:200).
    2. Anti-KV1.7 antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q17ST2
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 53948 Da
Additional Information
Gene ID 16495
Other Names Potassium voltage-gated channel subfamily A member 7, Voltage-gated potassium channel subunit Kv17, Kcna7, Kcnc7
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide TTRKAQEIHGKAPG(C), corresponding to amino acid residues 2-15 of mouse KV1.7 (Accession Q17ST2 ).ֲ ֲ Intracellular, N-terminus.The antibody does not recognize human Kv1.7.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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The Kv1.7 voltage-gated K+ channel, is a member of the Shaker family of K+ channels that today includes eight members (Kv1.1- Kv1.8). Kv1.7 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains and intracellular N and C termini. As with other channels of the Shaker subfamily, Kv1.7 can readily form heteromers with other members of the subfamily to compose the tetramer that forms the functional channel.   Kv1.7 mRNA was found to be expressed mainly in the heart with somewhat lower levels in pancreas and skeletal muscle.   The biophysical and pharmacological properties of the Kv1.7 channel closely resembles that of the ultra-rapidly activating delayed rectifier (IKur) in cardiac tissue. This current plays a central role in cardiac atrial repolarization that was largely believed to correspond to the activity of the Kv1.5 channel. This raises the possibility that the IKur current is the result of a heteromeric Kv1.5/ Kv1.7 channel.   We have now developed a highly specific antibody directed against the intracellular N-terminus domain of the Kv1.7 protein. Activity of the antibody was confirmed in mouse samples. The antibody will not recognize the human Kv1.7 protein.


References 1. Kalman, K. et al. (1997) J. Biol. Chem. 273, 5851. 2. Kashuba, V.I. et al. (2001) Gene 268, 115. 3. Bardien-Kruger, S. et al. (2002) Eur. J. Hum. Genet.10, 36.

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$ 475.00
$ 575.00
Cat# AG1113-050
(40 western blots)
Availability: 2-3 weeks
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