|Reactivity||Human, Mouse, Rat|
|Calculated MW||23885 Da|
|Homology||Mouse, human- identical.|
|Other Names||Calcium-activated potassium channel subunit beta-4, BK channel subunit beta-4, BKbeta4, Calcium-activated potassium channel, subfamily M subunit beta-4, Charybdotoxin receptor subunit beta-4, K(VCA)beta-4, Maxi K channel subunit beta-4, Slo-beta-4, Kcnmb4|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide AKLRVSYEYTEAEDKS(C), corresponding to amino acid residues 2-17 of rat slo־²4 (Accession Q9ESK8). Intracellular, N-terminal part.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
sloβ4 is a member of a family of regulatory b subunits that control the activity of the large conductance Ca2+-activated K+ channel KCa1.1. The family includes four members with a shared topology: two trans-membrane domains, short intracellular N- and C-termini and a large extracellular region. The four members of the family have a distinct tissue distribution with sloβ4 expressed almost exclusively in the central nervous sytem (CNS). Functionally, sloβ4 increases the sensitivity of the pore-forming KCa1.1 subunit to Ca2+ and voltage and it also changes its pharmacology. It has been shown that co-expression of sloβ4 with KCa1.1 makes the latter resistant to nM concentrations of the well-known inhibitors Charybdotoxin (#STC-325) and Iberiotoxin (#STI-400). The physiological significance of sloβ4 expression in the CNS is not clear, but KCa1.1 channels are likely involved in the regulation of neurotransmitter release in presynaptic terminals. We are pleased to introduce a new antibody directed against the intracellular N-terminus of the rat sloβ4. Anti-sloβ4 (KCNMB4) (#AG1115) will also recognize sloβ4 from human and mouse origin.
References 1. Meera, P. et al. (2000) PNAS 97, 5562. 2. Orio, P. et al. (2002) News Physiol. Sci. 17, 156. 3. Brenner, R. et al. (2000) J. Biol. Chem. 275, 6453.
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