|Homology||Mouse - 15/18 amino acid residues identical.|
|Other Names||CNG3, CNGα2, CCNC1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)GHGFSPDRENSEDASKAD, corresponding to amino acid residues 594-611 of rat CNGA3ֲ (accession number Q9QWN7). Intracellular, C-terminus.Also recognizes the two published isoforms of CNGA3.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Cyclic nucleotides are important second messengers in many cellular functions such as visual transduction, and relaxation of smooth muscle cells. Cyclic nucleotides exert their cellular functions through three major classes of cellular receptors, one of them is the cyclic nucleotide-gated (CNG) channels.1 The CNG channels are non-selective cation channels facilitating the influx of Na+ and Ca2+ ions, following activation by intracellular cyclic nucleotides.2 In vertebrates, six members of the CNG channel family were identified and grouped according to sequence homology into two subtypes, CNGA and CNGB. The native CNG channels are composed of a and b subunits in a tetrameric configuration. To date, four types of the a subunits (CNGA1-4) and two b subunits (CNGB1, CNGB3) have been characterized.3-4 Each subunit contains 6 TM domains and intracellular cAMP or cGMP binding domains but, are also modulated by other factors including phosphorylation and calmodulin.5 In a heterologous expression system, only the a subunits are capable of forming functional homomeric channels. CNG ion channels are essential in visual and olfactory signal transduction. The CNGA3 channel is primarily expressed in cone photoreceptors where it participates in light transduction (daylight and color vision). CNG channels expression was also found in the hippocampus, cortex, Purkinje cells and other neural derived tissues. CNGA3 was also detected in kidney, heart, brain and sperm, where it was thought to have an important role in the control of sperm motility and fertility. However, CNGA3-deficient mice were shown to be as fertile and produce at normal rate as wild types.6 Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of rat CNGA3. The Anti-CNGA3 antibody (#AG1116) can be used for western blot and immunohistochemical applications. It has been designed to recognize CNGA3 from rat and mouse samples.
References 1. Biel, M. et al.(1999) Rev. Physiol. Biochem. Pharmacol. 135, 151. 2. Kramer, R. H. and Molokanova, E. (2001) J. Exp. Biol. 204, 2921. 3. Matulef, K. (2003) Annu. Rev. Cell. Dev. Biol. 19, 23. 4. www.iuphar-db.org. 5. Molday, R.S. (1996) Curr. Opin. Neurobiol. 6, 445. 6. Biel, M.et al. (1999) Proc. Natl. Acad. Sci. USA. 96, 7553.
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