|Calculated MW||42605 Da|
|Homology||Guinea pig - 19/20 amino acid residues identical;human - 18/20 amino acid residues identical.|
|Other Names||ATP-sensitive inward rectifier potassium channel 15, Inward rectifier K(+) channel Kir42, Potassium channel, inwardly rectifying subfamily J member 15, Kcnj15|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EKQKLEEQYRQEDQRERELR, corresponding to amino acid residues 347-366 of mouse Kir4.2 (Accession O88932).ֲ ֲ Intracellular, C-terminal part.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.6 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Kir4.2 is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels. The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunit that can assembly as either homo or heteromers. Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis. Kir4.2 is a member of the Kir4 subfamily that includes one other member: Kir4.1. Kir4.2 can co-assemble with Kir4.1 but also with other Kir channels such as Kir1.1 and Kir5.1.2 The Kir4 subfamily has been classified as weak rectifiers with intermediate conductance. Kir4.2 is expressed in liver, kidney, pancreas lung and testis. Its physiological function is not well understood but it has been suggested that it could be involved in the regulation of K+ efflux in epithelial cells such as hepatocytes or lung cells.1
References 1. Hill, C.E. et al. (2002) Am J Physiol Gastrointest Liver Physiol 282,G233. 2. Pessia, M. et al. (2001) J Physiol 532,359.
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