|Calculated MW||86809 Da|
|Homology||Human, mouse - identical.|
|Other Names||Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3, Hcn3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)QRATGDGSPRRKGSGSER corresponding to amino acid residues 727-744 of rat HCN3 (Accession Q9JKA8). Intracellular, C-Terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain and play a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2 The Ih currents are generated by the Hyperpolarization-activated Cyclic Nucleotide-gated cannel family (HCN), which comprises of four homologous members, named HCN1-4. Each HCN subunit consist of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain to cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2 The HCN subunits can form functional homomers and can also co-assemble into functionally heteromers.2 The channels are closely related to each other and share homology of about 60%. However, they differ from each other in their cytoplasmic N- and the C-terminus. HCN1-4 mainly differ from each other with regard to their speed of activation and the extent by which they are modulated by cAMP. HCN1 is the fastest channel, followed by HCN2, HCN3 and HCN4.2,3 The HCN3 channels are abundant in the brain where high expression of HCN3 mRNA was found in the hypothalamus. Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of the HCN3 channel. The Anti-HCN3 (#AG1118) can be used for Western Blotting and Immunohistochemical applications. It has been designed to recognize HCN3 from human, rat and mouse samples.
References 1. Much, B. et al (2003) J.Biol.Chem. 278, 43781. 2. Notomi, T. and Shigemoto R. (2004) J. Comp. Neurol. 471, 241. 3. IUPHAR: http://www.iuphar-db.org/iuphar-rd/index.html.
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