Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   HCN1 Antibody   

HCN1 Antibody

Affinity purified polyclonal antibody

  • WB - HCN1 Antibody AG1119-025
    Western blot analysis of rat brain membrane:
    1. Anti-HCN1 antibody (#AG1119), (1:200).
    2. Anti-HCN1 antibody,  preincubated with the control peptide antigen.
  • IHC - HCN1 Antibody AG1119-025
    Expression of HCN1 in mouse cerebellum Immunohistochemical staining mouse cerebellum using Anti-HCN1 antibody (#AG1119). A. HCN1 (green) appears in the cerebellar pinceau. B. Calcium binding calbindinD28-K (red), a marker of Purkinje neurons, is stained in the same section. C. Merge of the images demonstrates the position of the HCN1-positive pinceau structures at the axon initial segment of Purkinje neurons.
  • ICC - HCN1 Antibody AG1119-025
    Expression of HCN1 in rat DRG primary culture
    Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat DRG primary culture. Cells were stained with Anti-HCN1 antibody (#AG1119), (1:300), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green).
    Nuclear staining of cells using the cell-permeable dye Hoechst 33342 (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9JKB0
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 102422 Da
Homology Mouse - 18/19 amino acid residues identical; human - 16/19 amino acid residues identical.
Additional Information
Gene ID 84390
Other Names Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, Hcn1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KPNSASNSRDDGNSVYPSK, corresponding to amino acid residues 6-24 of rat HCN1(Accession Q9JKB0). Intracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.85 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.


Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain and have a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2 The Ih currents are generated by the Hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which is comprised of four homologous members, HCN1-4. Each HCN subunit consists of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain for cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2 The HCN subunits can form functional homomers and can also co-assemble into functionally heteromers.2   The channels are closely related to each other and share a homology of about 60%. However, their similarity decreases in the cytoplasmic N- and C-termini. The channels HCN1-4 mainly differ from each other in their speed of activation and the extent to which they are modulated by cAMP.  HCN1, weakly affected by cAMP, is the fastest channel, followed by HCN2, HCN3 and HCN4.    HCN1 is extensively expressed in the brain, in specific areas like the neocortex, hippocampus, cerebellum and superior colliculus.2,3


References 1. Much, B. et al (2003) J.Biol.Chem. 278, 43781. 2. Notomi, T. and Shigemoto R. (2004) J. Comp. Neurol. 471, 241. 3. Gravante, B. et al (2004) J.Biol.Chem. 279, 43847.

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

$ 375.00
$ 475.00
$ 575.00
Cat# AG1119-025
(40 western blots)
Availability: 2-3 weeks
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609

Other Products

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Santa Cruz Alternative
Terms & Conditions