- CITATIONS: 1
|Calculated MW||59801 Da|
|Homology||Human - 19/20 amino acid residues identical.|
|Other Names||Potassium channel subfamily K member 10, Outward rectifying potassium channel protein TREK-2, TREK-2 K(+) channel subunit, Kcnk10, Trek2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide TFRNYSLDEEKKEDETEKMC, corresponding to amino acid residues 475-494 of rat K2P10.1 (accession number Q9JIS4). Intracellular, C-terminal domain.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Western blot analysis (WB): - Rat DRGs and plantar skin lysates (1:500) (see Acosta, C. et al. (2014) in Product Citations). Immunohistochemistry (IH): - Rat DRGs (1:500) (see Acosta, C. et al. (2014) in Product Citations). Immunocytochemistry (IC): - HEK-293 transfected cells (1:500) (see Acosta, C. et al. (2014) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
Provided below are standard protocols that you may find useful for product applications.
K2P10.1 is a member of the 2-pore (2P) domain K+ channels. These K+ channels also also known as background or “leak” channels and help to set the resting membrane potential. K2P channels can be activated by a wide variety of stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc. The channels can also be subclassified based on their specific activators. K2P10.1 can be integrated to a K2P subfamily which includes K2P2.1 (TREK1) and K2P4.1 (TRAAK) that are activated by intracellular unsaturated fatty acids such as arachidonic acid, low intracellular pH and mechanical stretch. K2P10.1 mRNA expression has been detected in the brain (primarily in the cerebellum) and in peripheral tissues such as pancreas and kidney.
References 1. Kim, D. (2003) Trends Pharmacol. Sci. 24, 648. 2. Lesage, F. et al. (2000) J. Biol. Chem. 275, 28398. 3. Bang, H. et al. (2000) J. Biol. Chem. 275, 17412.
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