|Reactivity||Human, Mouse, Rat|
|Calculated MW||93949 Da|
|Homology||Mouse- identical; human - 15/16 amino acid residues identical.|
|Other Names||Potassium voltage-gated channel subfamily KQT member 2, KQT-like 2, Potassium channel subunit alpha KvLQT2, Voltage-gated potassium channel subunit Kv72, Kcnq2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)RGPTITDKDRTKGPAE, corresponding to amino acid residues 578-593 of rat KCNQ2 (Accession number O88943). Intracellular, C-terminus.The antibody is specific for KCNQ2; it does not cross react with KCNQ3 or other QKT proteins. There are at least 9 recognized splice variants of rat KCNQ2. This antibody recognizes all except splice variants B and G.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Western blot analysis (WB): - Human SHSY-5Y cells (1:1000) (see Mucha, M. et al. (2010) in Product Citations). Immunohistochemistry (IH): - Guinea pig detrusor (1:200) (see Anderson, U.A. et al. (2013) in Product Citations). - Rat skin sections (0.7 µg/ml) (see Reilly, J.M. et al. (2013) in Product Citations). - Mouse optic nerve (see Bekku, Y. et al. (2010) in Product Citations). Immunocytochemistry (IC): - Rat cortical neurons (1:500) (see Peretz, A. et al. (2005) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The KCNQ family of voltage-gated K+ channels includes 5 known members: KCNQ1 to KCNQ5. Structurally, the KCNQ family belongs to the six transmembrane domain category of K+ channels. KCNQ family members can form either homomultimeric or heteromultimeric channels with different functional consequences. For example KCNQ2 and KCNQ3 heteromultimers give rise to a much larger channel current than when either protein is expressed alone, probably due to enhanced plasma membrane expression of the combined channel. Indeed, KCNQ2/KCNQ3 heteromultimers are believed to be the molecular correlates of the so-called M current. This current is a K+ neuronal current that is strongly inhibited by the activation of the M1 subtype of the muscarinic acetylcholine receptor. Mutations in either KCNQ2 or KCNQ3 are associated with a form of epilepsy known as benign familial neonatal convulsions (BNFC).
References 1. Kullmann, D.M. (2002) J. Mol. Brain 125, 1117. 2. Robbins, J. (2001) Pharmacol. Ther. 90, 1. 3. Wang, H.S. et al. (1998) Science 282, 1890.
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