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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   K2P2.1 (TREK-1) Antibody   

K2P2.1 (TREK-1) Antibody

Affinity purified polyclonal antibody

  • WB - K2P2.1 (TREK-1) Antibody AG1125-025
    Western blot analysis of rat brain membranes:
    1. Anti-K2P2.1 (TREK-1) antibody (#AG1125), (1:200).
    2. Anti-K2P2.1 (TREK-1) antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95069
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 47093 Da
Homology Rat - identical; mouse - 17/18 amino acidresidues identical.
Additional Information
Gene ID 3776
Other Names Potassium channel subfamily K member 2, Outward rectifying potassium channel protein TREK-1, TREK-1 K(+) channel subunit, Two pore domain potassium channel TREK-1, Two pore potassium channel TPKC1, KCNK2, TREK, TREK1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide DPKSAAQNSKPRLSFSTK(C), corresponding to residues 8-25 of human K2P2.1 (TREK-1) (Accession O95069).ֲ ֲ Intracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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K2P2.1 (also named TWIK-Related K+ channel, TREK-1 or KCNK2) is a member of the 2-pore (2P) domain K+ channels family that includes at least 16 members. These channels show little time or voltage dependence and are considered to be “leak” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.   The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini.    K2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators. K2P2.1 can be integrated to a K2P subfamily that includes K2P4.1 (TRAAK) and K2P10.1 (TREK2) that are activated by intracellular unsaturated fatty acids such as arachidonic acid, lysophosphatidic acid and mechanical stretch. In addition, K2P2.1 can also be activated by general anesthetics such as halothane and chloroform and intracellular acidification.   K2P2.1 expression in humans is largely restricted to the brain with some expression in ovary and small intestine while K2P2.1expression in rodents is more widespread.   K2P2.1 has an important role in mood regulation, as knockout mice show resistance to depression, suggesting that K2P2.1 may be a potential target for anti-depressants. K2P2.1 is involved in the fast release of glutamate from astrocytes. It requires the activation of Gαi, dissociation of Gβγ, followed by the opening of the glutamate permeable K2P2.1 (TREK-1) K+ channel through its interaction with Gβγ. For this purpose, TREK-1 is localized at the cell surface of the cell body and processes of astrocytes. Abgent is pleased to offer a highly specific antibody directed against an epitope of human K2P2.1. Anti-K2P2.1 (TREK-1) antibody (#AG1125) can be used in western blot analysis, and has been designed to recognize TREK-1 channel from mouse, rat and human samples.


References 1. Maingret, F. et al. (1999) J. Biol. Chem. 274, 26691. 2. Lesage, F. and Lazdunski, M. (2000) Am. J. Physiol. Renal Physiol.  279, F793. 3. Kim, D. (2003) Trends Pharmacol. Sci. 24, 648. 4. Heurteaux, C. et al. (2006) Nat. Neurosci. 9, 1134. 5. Woo, D.H. et al. (2012) Cell 151, 25.

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Cat# AG1125-025
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