|Calculated MW||76177 Da|
|Homology||Mouse - identical; rabbit - 15/18 amino acid residues identical; human, bovine - 14/18 amino acid residues identical.|
|Other Names||Cyclic nucleotide-gated olfactory channel, Cyclic nucleotide-gated cation channel 2, Cyclic nucleotide-gated channel alpha-2, CNG channel alpha-2, CNG-2, CNG2, Cyclic nucleotide-gated olfactory channel subunit OCNC1, Cnga2, Cncg2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)KQNHEDDYLSDGINTPEP, corresponding to amino acid residues 643-660 of rat CNGA2. (Accession Q00195). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Immunohistochemistry (IH): - Mouse olfactory cilia (1:200) (see Kaneko Goto, T. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Cyclic nucleotides are important second messengers in many cellular functions such as visual transduction, and relaxation of smooth muscle cells. Cyclic nucleotides exert their cellular functions through three major classes of cellular receptors, one of them is the cyclic nucleotide-gated (CNG) channels.1 The CNG channels are non-selective cation channels facilitating the influx of Na+ and Ca2+ ions, following activation by intracellular cyclic nucleotides.2 In vertebrates, six members of the CNG channel family were identified and grouped according to sequence homology into two subtypes, CNGA and CNGB. To date, four types of the a subunits (CNGA1-4) and two b subunits (CNGB1, CNGB3) have been characterized.3-4 Native CNG channels are composed of a and b subunits in a tetrameric configuration. Each subunit contains 6 TM domains and intracellular cAMP or cGMP binding domains, but are also modulated by other factors including phosphorylation and calmodulin.5 In a heterologous expression system, only the a subunits are capable of forming functional homomeric channels. CNG ion channels are essential in visual and olfactory signal transduction. CNG channels were originally detected in rod and cone photoreceptors and olfactory receptor cells, where they mediate the transduction of external sensory stimuli into neuronal activity.6 CNGA2 is predominantly expressed in olfactory neurons (the olfactory type receptor). However, electrophysiological and molecular data indicate that CNGA1, and especially CNGA2, are widely distributed and functionally active in many regions of the brain, including the hippocampus, cerebral cortex, cerebellum, and brainstem.7
References 1. Biel, M. et al.(1999) Rev. Physiol. Biochem. Pharmacol. 135, 151. 2. Kramer, R. H. and Molokanova, E. (2001) J. Exp. Biol. 204, 2921. 3. Matulef, K. (2003) Annu. Rev. Cell. Dev. Biol. 19, 23. 4. www.iuphar-db.org 5. Molday, R.S. (1996) Curr. Opin. Neurobiol. 6, 445. 6. Kingston, P.A. et al.(1996) Proc. Natl. Acad. Sci. USA. 93, 10440. 7. Podda, M.V. et al. (2005) Neuroreport, 16, 1939.
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