|Calculated MW||44357 Da|
|Homology||Human - 15/17 amino acid residues identical.|
|Other Names||Potassium channel subfamily K member 9, Acid-sensitive potassium channel protein TASK-3, TWIK-related acid-sensitive K(+) channel 3, Two pore potassium channel KT32, Two pore K(+) channel KT32, Kcnk9, Task3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)DDYQQLELVILQSEPHR, corresponding to amino acid residues 57-73 of rat K2P9.1 (accession number Q9ES08). Extracellular, near the P1 loop.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
K2P9.1 (also named TASK3 or KCNK9) is a member of the 2-pore (2P) domain K+ channels family that probably assemble as dimers to constitute a functional channel.1 These channels show little time or voltage dependence and are considered to be “leak” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The channels are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc.2 The physiological role of the K2P channels is not yet clear, though they were proposed to participate in breathing, aldosterone secretion and anesthetic-mediated neuronal activity. In human, expression of K2P9.1 is restricted in brain and its expression overlaps with the structurally related K2P3.1 (TASK1, KCNK3) channel. In rat, it is more widely expressed in other tissues as well as in brain. Unexpectedly, K2P9.1 was found to be over-expressed between 5 to 100-fold in 44% of breast cancer tumors tested. Transfection and over-expression of K2P9.1 in cell lines promotes tumor growth and confers resistance to hypoxia and serum deprivation.3 Recently, K2P9.1 was found to play an important role in K+-dependent apoptosis of cerebellar granules.4 Abgent is pleased to present a new polyclonal antibody to Anti-K2P9.1 (TASK3). The antibody was raised against an epitope corresponding to residues 57-73 of the rat K2P9.1 protein. The chosen epitope is located in the extracellular region near to the Pore 1 loop, and shares 15 out of 17 residues with the human isoform. Reactivity of the antibody was confirmed by Western blotting in both rat and human samples.
References 1. Lesage, F. and Lazdunski, M. (2000) Am J Physiol. 279, F793. 2. Patel, A.J. et al. (2001) Trends Neurosci. 24, 339. 3. Mu, D. et al. (2003) Cancer Cell 3, 297. 4. Lauritzen, I. et al. (2003) J. Biol. Chem. 278, 232068.
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