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Kir2.2 Antibody

Affinity purified polyclonal antibody

  • WB - Kir2.2 Antibody AG1128-050
    Western blot analysis of rat kidney (1,3) and brain (2,4) membranes: 
    1,2. Anti-Kir2.2 antibody (#AG1128), (1:200).   
    3,4. Anti-Kir2.2 antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P52188
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 48399 Da
Homology Mouse identical or 16/19 amino acid residues identical; human 13/19 amino acid residues identical.
Additional Information
Gene ID 117052
Other Names ATP-sensitive inward rectifier potassium channel 12, Inward rectifier K(+) channel Kir22, IRK-2, Potassium channel, inwardly rectifying subfamily J member 12, Kcnj12, Irk2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)EVATDRDGRSPQPEHDFDR,ֲ corresponding to amino acid residues 391-409 of rat Kir2.2 (Accession P52188). Intracellular, C-terminal part.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.39 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Citations (0)

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Kir2.2 is a member of the inward rectifying K+ channel family.   The Kir2 subfamily comprises 4 known members: Kir2.1, Kir2.2, Kir2.3 and Kir2.4.   The Kir2 subfamily members are strong inward rectifiers and are believed to be involved in maintaining the resting membrane potential and buffering of extracellular K+.   The Kir2.2 subunit is expressed in the CNS, kidney and heart where it was found to form heteromeric channels with Kir2.1.


References 1. Nichols, C.G. and Lopatin, A.N. (1997) Annu Rev Physiol. 59, 171. 2. Preisig-Muller, al. (2002) PNAS USA 99, 7774.

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$ 475.00
$ 575.00
Cat# AG1128-050
(40 western blots)
Availability: 2-3 weeks
Bulk Size
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