|Calculated MW||48399 Da|
|Homology||Mouse identical or 16/19 amino acid residues identical; human 13/19 amino acid residues identical.|
|Other Names||ATP-sensitive inward rectifier potassium channel 12, Inward rectifier K(+) channel Kir22, IRK-2, Potassium channel, inwardly rectifying subfamily J member 12, Kcnj12, Irk2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EVATDRDGRSPQPEHDFDR,ֲ corresponding to amino acid residues 391-409 of rat Kir2.2 (Accession P52188). Intracellular, C-terminal part.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.39 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Kir2.2 is a member of the inward rectifying K+ channel family. The Kir2 subfamily comprises 4 known members: Kir2.1, Kir2.2, Kir2.3 and Kir2.4. The Kir2 subfamily members are strong inward rectifiers and are believed to be involved in maintaining the resting membrane potential and buffering of extracellular K+. The Kir2.2 subunit is expressed in the CNS, kidney and heart where it was found to form heteromeric channels with Kir2.1.
References 1. Nichols, C.G. and Lopatin, A.N. (1997) Annu Rev Physiol. 59, 171. 2. Preisig-Muller, R.et al. (2002) PNAS USA 99, 7774.
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