|Calculated MW||33747 Da|
|Homology||Rat - 13/17 amino acid residues identical.|
|Other Names||Potassium channel subfamily K member 6, Inward rectifying potassium channel protein TWIK-2, TWIK-originated similarity sequence, KCNK6, TOSS, TWIK2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)ESHQQLSASSHTDYASIPR, corresponding to residues 295-313 of human K2P6.1 (TWIK-2) (Accession Q9Y257).ֲ ֲ Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.6 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
K2P6.1 (also named Tandem of P domain of Weak Inward rectifier K+ channel 2, TWIK-2 or KCNK6) is a member of the 2-pore (2P) domain K+ channel family that includes at least 16 members. These channels show little time or voltage dependence and are considered to be “leak” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini. The functional channel is believed to be a dimer. K2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators. K2P6.1 displays low single channel conductance of about 5pS and is partially activated by arachidonic acid and blocked by volatile anesthetics. K2P6.1 expression is widespread, especially in the rat were it is expressed in brain, heart, kidney and liver. In humans K2P6.1 expression is more restricted with expression in aorta, stomach, placenta and spleen while in mouse, K2P6.1 expression has been detected only in liver.
References 1. Chavez, R.A. et al. (1999) J. Biol. Chem. 274, 7887. 2. Patel, A.J. et al. (2000) J. Biol. Chem. 275, 28722. 3. Lesage, F. and Lazdunski, M. (2000) Am. J. Physiol. Renal Physiol. 279, F793.
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