|Calculated MW||59215 Da|
|Homology||Mouse - 17/19 amino acidresidues identical; human - 12/19 amino acid residues identical.|
|Other Names||Small conductance calcium-activated potassium channel protein 1, SK1, SKCa 1, SKCa1, KCa21, Kcnn1, Sk1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)DRPGSGKPPTVSHRLGHRR corresponding to amino acid residues 75-93 of rat KCa2.1 (Accession P70606). Intracellular, N-terminal part.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Western blot analysis (WB): - Canine LA and PV cardiomyocytes (1:200) (see Qi, X.Y. et al. (2014) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.6 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
KCa2.1 is a member of the Ca2+-activated K+ channels family with small conductance that includes KCa2.2 (SK2) and KCa2.3 (SK3). The channel is voltage insensitive and is activated by intracellular Ca2+ in the submicromolar range. It has a similar topology to that of voltage-dependent K+ channels (KV channels), that is six transmembrane domains and intracellular N- and C-termini. KCa2.1 channel expression is roughly confined to neuronal tissue, although there are some indications that it may be present in non-neuronal tissues as well. The sensitivity of KCa2.1 to the bee venom toxin Apamin (#STA-200) is a matter of some controversy. Expression of the human KCa2.1 in mammalian cell lines showed that most cells produce channels that can be blocked by Apamin. However, expression of KCa2.1 in Xenopus oocytes produced channels that were mostly insensitive to apamin. Interestingly, the rat KCa2.1 is unable to form functional channels when expressed alone in either cell lines or oocytes.
References 1. Sailer, C.A. et al. (2002) J. Neurosci. 22, 9698. 2. Monaghan, A.S. et al. (2004) J. Biol. Chem. 279, 1003.
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