|Application ||WB, IHC|
|Calculated MW||43965 Da|
|Homology||Mouse, human - identical.|
|Other Names||G protein-activated inward rectifier potassium channel 3, GIRK-3, Inward rectifier K(+) channel Kir33, Potassium channel, inwardly rectifying subfamily J member 9, Kcnj9, Girk3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)RLDAHLYWSIPSRLDEKV, corresponding to residues 344-361 of rat Kir3.3 (Accession Q63511). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||3 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Kir3.3 (or G-protein regulated Inward-Rectifier K+ channel, GIRK3) is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels. The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunit that can assembly as either homo or heterotetramers. Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis. Kir3.3 is a member of the Kir3.x subfamily that includes four members (Kir3.1- Kir3.4). The Kir3 family is characterized by the fact that the channels can be activated by neurotransmitters and other factors acting via the activation of G-protein coupled receptors. Binding of the corresponding ligand to the G-protein receptor induces the dissociation of Ga-GTP from the Gbg dimer. The latter directly binds to Kir3 and activates the channel.1,2 Kir3.3 is mainly expressed in the brain, were it co-assembles with Kir3.1 or Kir3.2. The functional impact of Kir3.3 is less well understood than the other Kir3 channels. However, heteromers composed of Kir3.2 and Kir3.3 were found to be primarily responsible for the opioid-induced current and hyperpolarization observed in mouse locus ceruleus (LC) neurons.3
1. Dascal, N. (1997) Cell Signal 9, 551–573.
2. Mark, M.D. et al. (2000) Eur. J. Biochem. 267, 5830.
3. Torrecilla, M. et al. (2002) J Neurosci. 22, 4328.
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