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>   home   >   Products   >   Primary Antibodies   >   Kir3.3 (GIRK3) Antibody   

Kir3.3 (GIRK3) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Kir3.3 (GIRK3) Antibody AG1131-050
    Western blot analysis of rat brain membranes: 1. Anti-Kir3.3 (GIRK3) antibody (#AG1131), (1:200). 2. Anti-Kir3.3 (GIRK3) antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q63511
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 43965 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 116560
Other Names G protein-activated inward rectifier potassium channel 3, GIRK-3, Inward rectifier K(+) channel Kir33, Potassium channel, inwardly rectifying subfamily J member 9, Kcnj9, Girk3
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)RLDAHLYWSIPSRLDEKV, corresponding to residues 344-361 of rat Kir3.3 (Accession Q63511).  Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.75 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 3 µg peptide per 1 µg antibody.
Citations (0)

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Kir3.3 (or G-protein regulated Inward-Rectifier K+ channel, GIRK3) is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels. The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunit that can assembly as either homo or heterotetramers. Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis. Kir3.3 is a member of the Kir3.x subfamily that includes four members (Kir3.1- Kir3.4). The Kir3 family is characterized by the fact that the channels can be activated by neurotransmitters and other factors acting via the activation of G-protein coupled receptors. Binding of the corresponding ligand to the G-protein receptor induces the dissociation of Ga-GTP from the Gbg dimer. The latter directly binds to Kir3 and activates the channel.1,2 Kir3.3 is mainly expressed in the brain, were it co-assembles with Kir3.1 or Kir3.2. The functional impact of Kir3.3 is less well understood than the other Kir3 channels. However, heteromers composed of Kir3.2 and Kir3.3 were found to be primarily responsible for the opioid-induced current and hyperpolarization observed in mouse locus ceruleus (LC) neurons.3


1. Dascal, N. (1997) Cell Signal 9, 551–573.
2. Mark, M.D. et al. (2000) Eur. J. Biochem. 267, 5830.
3. Torrecilla, M. et al. (2002) J Neurosci. 22, 4328.

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$ 475.00
$ 575.00
Cat# AG1131-050
(40 western blots)
Availability: 2-3 weeks
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