|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||47783 Da|
|Homology||Mouse, human, pig - identical.|
|Other Names||G protein-activated inward rectifier potassium channel 4, GIRK-4, Cardiac inward rectifier, CIR, Heart KATP channel, Inward rectifier K(+) channel Kir34, KATP-1, Potassium channel, inwardly rectifying subfamily J member 5, Kcnj5, Girk4|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide RNAMNQDMEIGVT(C), corresponding to amino acid residues 6-18 of rat Kir3.4 (Accession P48548). Intracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Immunohistochemistry (IH): - Mouse, rat and human heart sections (1:100) (see Liang, B. et al. (2014) in Product Citations). Also tested in mouse GIRK4-/- - Rat heart sections (1:20) see Atkinson, A.J. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.025% NaN3.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Kir3.4 is a member of the G-protein regulated inward-rectifier K+ (GIRK) channel subfamily which is part of an inward-rectifier K+ channel superfamily. The GIRK subfamily comprises four members in mammals (Kir3.1- Kir3.4) that present the common topology of the inward-rectifier superfamily: two transmembrane domains flanking a highly conserved pore region with the N and C-terminus located intracellularly. Kir3.4 and the other Kir3 family subunits can be activated by neurotransmitters and other factors via the activation of G-protein coupled receptors. Binding of the corresponding ligand to the G-protein receptor induces the dissociation of G α-GTP from the Gβγ dimer. The latter directly binds to Kir3 and activates the channel. Kir3.4 expression is largely confined to the heart, where it co-assembles with Kir3.1 to form the prototypical muscarinic-gated K+ channel KACh. Indeed, knockout mice for Kir3.4 showed impaired heart rate following vagal nerve stimulation. A peptide toxin originating from the Apis mellifera bee venom, Tertiapin (#STT-250) was shown to be a potent blocker of Kir3.4 containing channels (8.6 nM for the Kir3.1/3.4 combination).4
1. Mark, M.D. Herlitze, S. (2000) Eur. J. Biochem. 267, 5830
2. Yamada, M. et al. (1998) Pharmacol. Rev. 50, 723.
3. Wickman, K. et al. (1998) Neuron 20, 103.
4. Jin, W. and Lu, Z. (1998) Biochemistry 37, 13291.
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