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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   KCa2.3 (N-term) Antibody   

KCa2.3 (N-term) Antibody

Affinity purified polyclonal antibody

     
  • WB - KCa2.3 (N-term) Antibody AG1139-025
    Western blot analysis of rat brain membranes:
    1. Anti-KCa2.3 (SK3) (N-term) antibody (#AG1139), (1:200).
    2. Anti-KCa2.3 (SK3) (N-term) antibody, preincubated with the control peptide antigen.
  • IHC - KCa2.3 (N-term) Antibody AG1139-025
    Expression of KCa2.3 in mouse dopaminergic neurons Immunohistochemical staining of mouse dopaminergic neurons using Anti-KCa2.3 (SK3) (N-term) antibody (#AG1139). A. KCa2.3 is detected in substantia nigra pars compacta. B. Tyrosine hydroxylase staining shows dopaminergic neurons.  Triangles point at cells with co-localization.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9UGI6
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 82026 Da
Homology Rat, mouse, pig - identical.
Additional Information
Gene ID 3782
Other Names Small conductance calcium-activated potassium channel protein 3, SK3, SKCa 3, SKCa3, KCa23, KCNN3, K3
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide DTSGHFHDSGVGDLDEDPKC, corresponding to amino acid residues 2-21 of human KCa2.3 (SK3) (Accession Q9UGI6). Intracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Application Details Western blot analysis (WB): - Human iPS cells (1:500) (see Linta, L. et al. (2013) in Product Citations). - Mouse and human uterine tissue (1:100) (see Pierce, S.L. and England, S.K. (2010) in Product Citations). - Rat mesenteric arteries (see Hilgers, R.H. et al. (2010) in Product Citations). Immunohistochemistry (IH): - Mouse VNO neurons, also tested in conditional KO mice (see Kim, S. et al. (2012) in Product Citations). Rat mesenteric artery parafin-embedded sections (see Hilgers, R.H. et al. (2010) in Product Citations). Immunocytochemistry (IC): - Human iPS cells (1:100) (see Linta, L. et al. (2013) in Product Citations). - CHO-K1 cells expressing human SK3 (1:1000) (see Terstappen, G.C. et al. (2001) in Product Citations).  
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.6 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
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Background

KCa2.3 is a member of the Ca2+-activated K+ channels family with small conductance that includes KCa2.1 (SK1) and KCa2.2 (SK2). The channel is voltage insensitive and is activated by intracellular Ca2+ in the submicromolar range. It has, though, a similar topology to that of voltage-dependent K+ channels (Kv channels), that is six transmembrane domains and intracellular N- and C-termini. The functional channel of all the KCa2 family members is a multimeric protein composed of four pore-forming subunits. KCa2 channels are extremely sensitive to the levels of intracellular Ca2+ and concentrations as low as 300-700 nM can open the channels very rapidly (5-15 ms). Hence, the KCa2 channels are highly sensitive and fast Ca2+ sensors resembling other known Ca2+-binding proteins. This type of Ca2+-dependent activation is achieved by the constitutive binding of the KCa2 channels to calmodulin, a highly expressed Ca2+-binding protein via a calmodulin-binding domain situated at the cytoplasmic C-termini.   Pharmacologically, the KCa2 channels are the only known targets of the bee venom toxin Apamin, with KCa2.1 being the less sensitive, KCa2.2 the most sensitive and KCa2.3 showing intermediate sensitivity. KCa2.3 is predominantly expressed in the nervous system although expression in endothelial cells, heart and liver have been described. KCa2.3 is known to be involved in the regulation of neuronal excitability. They do so mainly via a phenomenon known as afterhyperpolarization in which KCa2 channels open in response to increased intracellular Ca2+ concentrations that result from the entry of extracellular Ca2+ through voltage-dependent Ca2+ channels during action potentials. In this way, KCa2 channels effectively form a Ca2+-mediated feedback loop. KCa2.3 is involved in the control of firing rate and subsequent dopamine secretion from midbrain dopaminergic neurons. Since malfunction of these neurons is involved in several pathological disorders such as Parkinson’s disease and schizophrenia, modulators of the KCa2.3 channels have been proposed to be of therapeutic value in these diseases.

References

References 1. Kohler, M. et al. (1996) Science 273, 1709. 2. Xia, X.M. et al. (1998) Nature 395, 503. 3. Stocker, M. (2004) Nat. Rev. Neurosci. 5, 758.

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$ 375.00
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$ 575.00
Cat# AG1139-025
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(40 western blots)
Availability: 2-3 weeks
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