|Calculated MW||43518 Da|
|Homology||Rat, mouse - 17/18 amino acidresidues identical. Homology with human TASK-3: 11/17 amino acid residues identical.|
|Other Names||Potassium channel subfamily K member 3, Acid-sensitive potassium channel protein TASK-1, TWIK-related acid-sensitive K(+) channel 1, Two pore potassium channel KT31, Two pore K(+) channel KT31, KCNK3, TASK, TASK1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EDEKRDAEHRALLTRNGQ, corresponding to amino acid residues 252-269 of human K2P3.1 (TASK-1) (Accession O14649).ֲ ֲ Intracellular, C-terminal part.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
K2P3.1 (also named TWIK-Related Acid Sensitive K+ channel, TASK-1 or KCNK3) is a member of the 2-pore (2P) domain K+ channels family that includes at least 16 members. These channels show little time or voltage dependence and are considered to be “leak” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini. The functional channel is believed to be a dimer. K2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators. K2P3.1 is extremely sensitive to variations in external pH, a drop in pH from 7.7 to 6.7 will be enough to close most K2P3.1 channels. K2P3.1 channel distribution is relatively wide with high expression in the brain and heart as well as in the pancreas, kidney and lung. K2P3.1 can exist in the brain either as a homodimer or as a heterodimer with the closely related channel K2P9.1 (TASK-3). During periods of high neuronal activity a transient increase in extracellular pH has been recorded. This pH increase may stimulate K2P3.1 channel activity and hence enhance outward K+ currents. This in turn will decrease excitability in these neurons and work as a feedback mechanism.
References 1. Duprat, F. et al. (1997) EMBO J. 16, 5464. 2. Lesage, F. and Lazdunski, M. (2000) Am. J. Physiol. Renal Physiol. 279, F793. 3. Bayliss, D.A. et al. (2003) Mol. Interv. 3, 205.
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