|Application ||WB, IP|
|Calculated MW||70549 Da|
|Homology||Mouse, human - 15/16 amino acid residues identical.|
|Other Names||Potassium voltage-gated channel subfamily D member 2, RK5, Shal1, Voltage-gated potassium channel subunit Kv42, Kcnd2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)SNQLQSSEDEPAFVSK, corresponding to amino acid residues 454-469 of rat Kv4.2 (Accession Q63881). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.6 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Kv4.2 is a voltage-dependent K+ channel that belongs to the Shal channel subfamily and includes two other members: Kv4.1 and Kv4.3.1 Kv4.2 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. As with other members of the voltage-gated K+ channel superfamily, the functional channel is a tetramer that can be composed of more than one member of the Shal subfamily, i.e. heterotetramers of Kv4.1 and Kv4.3. The Kv4 channels are characterized by activation at subthreshold membrane potentials, inactivate rapidly and recover from inactivation quickly compared with other voltage-dependent K+ channels. This type of current is known as transient A-type K+ currents. For example, depolarization-activated K+ currents in rat neostriatal cholinergic interneurons are predominantly of the A-type and attributable to coexpression of Kv4.2 and Kv4.1 subunits.2 The biophysical properties of the Kv4.2 subunit can be modified by its association with auxiliary ß subunits such as the KChIP family that increase Kv4.2 current densities and accelerates both the inactivation and the recovery time. Kv4.2 is also highly expressed in the heart where together with Kv4.3 underlie the fast inactivating and recovering cardiac transient outward current Ito.3 Several toxins from spider venoms are potent blockers (affecting the channels in the nanomolar range) of Kv4.2 channels. Among these the most potent and selective are Stromatoxin-1 (#STS-350), (1.2nM), Phrixotoxin-1 (#P-710), (5nM), Phrixotoxin-2 (#P-700), (34nM) and Heteropodatoxin-2 (#STH-340), (100nM).4 Abgent is pleased to offer a highly specific antibody directed against an epitope of rat Kv4.2. Anti-Kv4.2 antibody (#AG1141) can be used in western blot, immunoprecipitation, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize KV4.2 from human, rat and mouse samples.
References 1. Roberds, S.L. and Tamkun, M.M. (1991) Proc Natl Acad Sci USA 88,1798. 2. Serodio, P. et al. (1994) J Neurophysiol 72,1516. 3. Guo, W. et al. (2002) Circ Res 90, 586. 4. Escoubas, P. et al. (2002) Mol. Pharmacol. 62, 48.
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