|Application ||WB, IP|
|Homology||Chicken - 91/99 amino acid residues identical; rabbit - 88/99 amino acid residues identical;turtle - 87/99 amino acid residues identical.|
|Other Names||BKCa; KCNMA1; SLO|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||GST fusion protein with the sequence SHSSHSSQ SSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMTRMG QAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIP PIREVEDEC, corresponding to residues 1097-1196 of mouse KCa1.1 variant 2 (Accession Q08460-2). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by DNA sequence and SDS-PAGE.|
|Application Details||Western blot analysis (WB): - Rat mesenteric arteries (1:300) (see Shi, L.1 et al. (2013) in Product Citations). Immunoprecipitation (IP): - Rat brain lysates (2 μg) (see Park, S.M. et al. (2004) in Product Citations). Immunohistochemistry (IH): - Rat mesenteric arteries (1:200) (see Shi, L. et al. (2013) in Product Citations). - Mouse vomeronasal sections (see Zhang, P. et al. (2008) in Product Citations). Immunocytochemistry (IC): - Mouse vomeronasal neurons (see Zhang, P. et al. (2008) in Product Citations),|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.4 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl PBS.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||3 µg fusion protein per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.025% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The KCa1.1 channel (also known as BKCa, Maxi K+ or slo) is part of a structurally diverse group of K+ channels that are activated by an increase in intracellular Ca2+. KCa1.1 shows a large single channel conductance when recorded electrophysiologically and hence its name. It differs from the rest of the subfamily members in that it can be activated by both an increase in intracellular Ca2+ and by membrane depolarization. In addition, the KCa1.1 channel structurally differs from the other Ca2+-dependent K+ channels. While the latter group has a topology that resembles that of the voltage-dependent K+ channels, the KCa1.1 channel has an extracellular N-terminus domain as well as an additional transmembrane domain. KCa1.1 is expressed in virtually all cell types where it causes hyperpolarization and helps to connect between intracellular Ca2+ signaling pathways and membrane excitability. Indeed, KCa1.1 channels play a crucial role in smooth muscle contractility, neuronal spike shaping and neurotransmitter release.
References 1. Wallner, M. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 4137. 2. Xia, X.M. et al. (1999) J. Neurosci. 19, 5255. 3. Orio, P. et al. (2002) News Physiol. Sci. 17, 156.
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