|Calculated MW||43608 Da|
|Homology||Mouse- identical; human - 12/14 amino acid residues identical.|
|Other Names||ATP-sensitive inward rectifier potassium channel 11, BIR, Inward rectifier K(+) channel Kir62, Potassium channel, inwardly rectifying subfamily J member 11, Kcnj11|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)SVAVAKAKPKFSIS,ֲ corresponding to amino acid residues 372-385 of rat Kir6.2ֲ ֲ (accession number P70673). Intracellular, C-terminal part.The antibody is specific for Kir6.2, it does not cross react withKir6.1.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Western blot analysis (WB): - Human placenta and pancreas lysate (see Lybaert, P. et al. (2013) in Product Citations). - Rat primary adrenomedullary chromafin cells (AMCs), (1:1000) (see Salman, S. et al. (2013) in Product Citations). Immunohistochemistry (IH): - Human placenta sections (see Lybaert, P. et al. (2013) in Product Citations). Immunocytochemistry (IC): - Mouse ciliary cells (1:100) (see Ohba, T. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.6 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||2 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.025% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Kir6.2 is a member of the inward rectifier K+ channels (Kir channels), a large family of voltage-independent K+ channels largely involved in stabilization of the membrane resting potential and in K+ transport across membranes. Kir channels can be modulated by a variety of intracellular agents such as protons, GTP-binding proteins and adenine nucleotides. The ATP-sensitive channel (KATP) is especially important since it couples cellular metabolism (intracellular ATP levels) with cell excitability. KATP channels have been described in pancreatic b-cells, neurons, heart, skeletal and smooth muscle. The KATP channel is composed of a Kir6.2 or Kir6.1 subunit and a sulphonylurea receptor (SUR) subunit. The pancreatic KATP channel for example, is composed of a complex of Kir6.2 and SUR1 subunits, while the cardiac KATP channel is composed of Kir6.2 and SUR2A complexes. Impaired b-cell KATP channel function due to mutations in either Kir6.2 or SUR1 subunits has been linked to the recessive autosomal disorder called persistent hyperinsulinemic hypoglycemia of infancy (PHHI). In addition, a Kir6.2 variant has recently been linked to an increased risk of developing type-2 diabetes.
References 1. Babenko, A.P. et al. (1998) Annu. Rev. Physiol. 60, 667. 2. Huopio, H. et al. (2002) Am. J. Physiol. 283, 207.
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