|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||68435 Da|
|Homology||Human - Identical.|
|Other Names||Potassium voltage-gated channel subfamily C member 4, Raw3, Voltage-gated potassium channel subunit Kv34, Kcnc4|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide EAGDD ERELA LQRLG PHEG(C), corresponding to amino acid residues 177-195 of rat Kv3.4 (Accession Q63734). Intracellular, N-terminal.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.3 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Kv3.4 is a member of the voltage-gated K+ channel superfamily. Together with the related proteins Kv3.1, Kv3.2 and Kv3.3 they constitute the Shaw type subfamily family.1 As with all Kv channels, Kv3.4 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. The functional Kv channel is a tetramer that can either be a homomer or a heteromer of Kv3 subunits.2 Kv3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of action potentials and to facilitate repetitive high frequency firing.2 Kv3.4 expression is wide and the channel can be found in brain, skeletal muscle, prostate and pancreas among others. Kv3.4 subunits have been implicated recently in the response mechanism to chronic hypoxia and the etiology of Alzheimer's and Parkinson's diseases. In addition, Kv3.4 was found to associate with the auxiliary subunit KCNE3 (MirP2) in skeletal muscle. A mutation in KCNE3 (R83H) has been associated with an inherited form of periodic paralysis (Thyrotoxic hypokalemic periodic paralysis) that is caused by the altered physiological function of the Kv3.4 channel.3 Toxins from sea anemone, BDS-I (#B-400), (47nM) and BDS-II (#B-450), (56nM), are potent blockers of KV3.4 channels.4
References 1. Schroter, K.H. et al. (1991) FEBS Lett. 278, 211. 2. Rettig, J. et al. (1992) EMBO J. 11, 2473. 3. Abbott, G.W. et al. (2001) Cell 104, 217. 4. Diochot, S. et al. (1998) J. Biol. Chem. 273, 6744.
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