|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Homology||Rat, rabbit - identical; mouse - 16/17 amino acid residues identical.|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)NEALELTGTPEEEHMGK, corresponding to amino acid residues 451-468 of human Kv4.3 (Accession O60577). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Kv4.3 is a voltage-dependent K+ channel that belongs to the Shal channel subfamily and includes two other members: Kv4.1 and Kv4.2. Kv4.3 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. As with other members of the voltage-gated K+ channel superfamily, the functional channel is a tetramer that can be composed of more than one member of the Shal subfamily, i.e. heterotetramers of Kv4.1 and Kv4.2. The Kv4 channels are characterized by activation at subthreshold membrane potentials, inactivate rapidly and recover from inactivation quickly compared with other voltage-dependent K+ channels. This type of current is known as transient A-type K+ currents.1 The biophysical properties of the Kv4.3 subunit can be modified by its association with auxiliary ß subunits such as the KChIP family that increase current densities and accelerates both the inactivation and the recovery time. Kv4.3 is highly expressed in the brain where it has a key role in shaping the action potential and firing frequency of neurons. In the heart together with Kv4.2 it underlies the fast inactivating and recovering cardiac transient outward current Ito.2,3 The channel is also expressed in smooth muscle cells of several organs such as myometrium, lung and colon where its function has not been completely elucidated. Several toxins from spider venoms are potent blockers (affecting the channels in the nanomolar range) of KV4.3 channels. Among these the most potent and selective are Phrixotoxin-1 (28 nM) and Phrixotoxin-2 (#P-700), (71 nM). 4
References 1. Serodio, P. et al. (1994) J. Neurophysiol. 72,1516. 2. Tsaur, M.L. et al. (1997) FEBS Lett. 400, 215. 3. Guo, W. et al. (2002) Circ. Res. 90, 586. 4. Diochot, S. et al. (1999) Br. J. Pharmacol. 126, 251.
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