|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||70191 Da|
|Homology||Human - 19/21 amino acid residues identical (based on ESTs).|
|Other Names||Potassium voltage-gated channel subfamily C member 2, KSHIIIA, Voltage-gated potassium channel subunit Kv32, Kcnc2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide DLGGKRLGIEDAAGLGGPDGK(C), corresponding to amino acid residues 184-204 of rat KV3.2 (Accession P22462). Intracellular, N-terminal part.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.3 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Kv3.2 is a member of the voltage-gated K+ channel superfamily. Together with the related proteins Kv3.1, Kv3.3 and Kv3.4 they constitute the Shaw type subfamily family.1 As with all Kv channels, Kv3.2 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. The functional Kv channel is a tetramer that can either be a homomer or a heteromer of Kv3 subunits. Kv3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of action potentials and to facilitate repetitive high frequency firing.2,3 Kv3.2 is highly expressed in the brain but has been also detected in peripheral organs such as pancreas and mesenteric artery. Kv3.2 and Kv3.1 are highly enriched in neurons that fire at high frequencies, such as fast spiking interneurons of the cortex and hippocampus and neurons in the globus pallidus. Their unusually rapid activation and deactivation rates allow channels containing Kv3.2 and Kv3.1 subunits to repolarize action potentials quickly thus minimizing the rate of recovery of sodium channel inactivation.2,3
References 1. Rettig, J. et al. (1992) EMBO J. 11, 2473. 2. Rudy, B. et al. (1999) Ann. NY Acad. Sci. 868, 304. 3. Rudy, B. et al. (2001) Trends Neurosci. 24, 517.
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