|Reactivity||Human, Mouse, Rat|
|Calculated MW||56701 Da|
|Homology||Mouse, dog, human, - identical.|
|Other Names||Potassium voltage-gated channel subfamily A member 2, RAK, RBK2, RCK5, Voltage-gated potassium channel subunit Kv12, Kcna2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||GST fusion protein with sequence YHRETEGEEQAQYLQVTSCPKIPSSPDLKK SRSASTISKSDYMEIQEGVNNSNEDFREENLKTANCTLANTNYVNITKMLTDV, corresponding to amino acid residues 417-499 of rat KV1.2 (Accession P63142).ֲ ֲ Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by DNA sequence and SDS-PAGE.|
|Application Details||Western blot analysis (WB): - Mouse spinal cord lysate (1:1000) (see Zoupi, L. et al. (2013) in Product Citations). - Rat lung lysate (see Lv, Y. et al. (2013) in Product Citations). Immunohistochemistry (IH): - Mouse spinal cord and cortex sections (1:200) (see Zoupi, L. et al. (2013) in Product Citations). - Mouse cerebellum (1:300) (see Kleopa, K.A. et al. (2006) in Product Citations). Immunocytochemistry (IC): - HeLa transfected cells (1:200) (see Kleopa, K.A. et al. (2006) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl PBS.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||3 µg fusion protein per 1 µg antibody.|
|Formulation||Lyophilized powder. Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
KV1.2 is a mammalian voltage dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.2 was first cloned from rat brain.1 Eight Shaker related genes exist in mammals constituting the KV1, subfamily of the large KV channel family of genes.2 A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function. The structure of KV1.2 channel is similar to all KV channels and includes six membrane spanning helixes creating a voltage sensor domain and a pore domain. 2 The channel is expressed in neurons and cardiac and smooth muscle tissue as well as in retina and pancreas.2 The crystal structure of KV1.2 was recently solved shading light on the structure of a mammalian voltage dependent channel.3 The functional channel is considered low voltage activated and shows very little inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle. KV1.2 channels are sensitive to high doses of TEA (560 mM) and low doses of 4-AP (0.59 mM), the “classical” non-selective potassium channel blockers. Several venomous toxins from Snakes, Scorpions and Honey Bee are potent blockers (affecting the channels in the nanomolar range) of KV1.2 channels. Among these the most potent and selective are α-Dendrotoxin (#D-350, 1-12 nM), Dendrotoxin-I (#D-390, 0.13 nM), Maurotoxin (STM-340, 0.1-0.8 nM), Hongotoxin-1 (#RTH-400, 0.17 nM), Margatoxin (#RTM-325, 0.16-0.65 nM), Tityustoxin Kα (#STT-360, 0.21 nM) and MCD peptide (STM-250, 10-400 nM).4
References 1. McKinnon, D. (1989) J.Biol. Chem. 264, 8230. 2. Gutman, G.A. et al. (2005) Pharmacol. Rev. 57, 473. 3. Long, S.B. et al. (2005) Science 309, 897. 4. Bogin, O. (2006) Modulator 21, 28.
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