|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||66580 Da|
|Homology||Rat - 86/90 amino acid residues identical; rabbit - 71/90 amino acid residues identical; human - 70/90 amino acid residues identical; bovine, dog - 66/90 amino acid residues identical.|
|Other Names||Potassium voltage-gated channel subfamily A member 5, Voltage-gated potassium channel subunit Kv15, KV1-5, Kcna5|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||GST fusion protein with sequence HRETDHEEQAALKEEQGIQRRESGLDTGGQRKVSCSKASFHKTGGPLEST DSIRRGSCPLEKCHLKAKSNVDLRRSLYALCLDTS RETDL, corresponding to amino acid residues 513-602 of mouse KV1.5 (Accession Q61762), (MW: 37 kDa.). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by DNA sequence and SDS-PAGE.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.6 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl PBS.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||3 µg fusion protein per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
KV1.5 is a mammalian voltage dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.5 was first cloned from rat brain.1 Eight Shaker related genes exist in mammals constituting the KV1, subfamily of the large KV channel family of genes.2 A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function. The structure of KV1.5 channel is similar to all KV channels and includes six membrane spanning helixes creating a voltage sensor domain and a pore domain. 2 The channel is expressed in cardiac and smooth muscle tissue (colon, aorta, stomach and pulmonary artery) as well as in neurons and kidney.2 A loss of function mutation in the gene encoding the channel was found in atrial fibrilation patients, stressing its role as a cardiac action potential regulator.3 The functional channel is considered transient (A-type) channel and shows prominent inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle. KV1.5 channels are sensitive to high doses of TEA (330 mM) and low doses of 4-AP (0.27 mM), the “classical” non-selective potassium channel blockers.2
References 1. Swanson, R. et al. (1990) Neuron 4, 929. 2. Gutman, G. A. et al. (2005) Pharmacol. Rev. 57, 473. 3. McGahon, M. K. et al. (2007) Am. J. Physiol. Heart Circ. Physiol. 292, H1001.
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