|Calculated MW||58884 Da|
|Homology||Mouse - 67/68 amino acid residues identical; human - 63/68 amino acid residues identical.|
|Other Names||Potassium voltage-gated channel subfamily A member 6, RCK2, Voltage-gated potassium channel subunit Kv16, Voltage-gated potassium channel subunit Kv2, Kcna6|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||GST fusion protein with a sequence NYFYHRETEQEEQGQYTHVTCGQPTPDLKATDNGLGKPDFAEASRERRSSYLPTPHRAYAEKRMLTEV, corresponding to amino acid residues 463-530 of rat Kv1.6 (Accession P17659), (MW: 35 kDa.). Intracellular, C-terminus.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.3 mg/ml.|
|Buffer After Reconstitution||phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl PBS.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||3 µg fusion protein per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
KV1.6 is a mammalian voltage dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.6 was first cloned from human brain.1 Eight Shaker related genes exist in mammals constituting the KV1, subfamily of the large KV channel family of genes.2 A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function. The structure of KV1.6 channel is similar to all KV channels and includes six membrane spanning helixes creating a voltage sensor domain and a pore domain. 2 The channel is expressed in neurons and other supporting cells in the brain, in cardiac and smooth muscle tissue as well as in ovary and testis2 and its activity influences the membrane potential and excitability of expressing cells. KV1.6 channels are sensitive to low doses of TEA (7 mM) and high doses of 4-AP (1.5 mM), the “classical” non-selective potassium channel blockers. Several toxins from snakes, scorpions and sea anemones venoms are potent blockers (affecting the channels in the nanomolar range) of KV1.6 channels. Among these the most potent and selective are α-Dendrotoxin (#D-350), (9-25 nM) and δ-Dendrotoxin (#D-380), (23 nM), Agitoxin-2 (#RTA-420), (0.036 nM) Hongotoxin-1 (#RTH-400), (6 nM), Margatoxin (#RTM-325), (5 nM) and Stichodactyla Toxin (#RTS-400), (0.16 mM).3 Abgent is pleased to offer a highly specific antibody directed against an epitope of rat Kv1.6. Anti-Kv1.6 antibody (#AG1153) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize KV1.6 from human, rat and mouse samples.
References 1. Grupe, A. et al. (1990) EMBO J. 9, 1749. 2. Gutman, G. A. et al. (2005) Pharmacol. Rev. 57, 473. 3. Bogin, O (2006) Modulator 21, 28.
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