|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||63842 Da|
|Homology||Rat, rabbit, mouse - identical.|
|Other Names||Potassium voltage-gated channel subfamily A member 3, HGK5, HLK3, HPCN3, Voltage-gated K(+) channel HuKIII, Voltage-gated potassium channel subunit Kv13, KCNA3, HGK5|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||GST fusion protein with sequence TLSKSEYMVIEEGGMNHSAFPQTPFKTGNSTATCTTNNNPNSCVNIKKIFTDV, corresponding to amino acid residues 523-575 of human Kv1.3 (Accession P22001). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by DNA sequence and SDS-PAGE.|
|Application Details||Western blot analysis (WB): - Mouse activated macrophages (BMDM cells) (see Moreno, C. et al. (2013) in Product Citations). - Human CD3+ cells (see Szigligeti, P. et al. (2006) in Product Citations). Immunoprecipitation (IP): - Transfected HEK-293 cell lysate (see Vicente, R. et al. (2006) in Product Citations). Immunocytochemistry (IC): - Human peripheral blood mononuclear cells (PBMCs) (see Hu, L. et al. (2013) in Product Citations). - Rat neural progenitor cells (NPCs) (1:200) (see Liebau, S. et al. (2006) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.6 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl PBS.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||3 µg fusion protein per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
KV1.3 belongs to the Shaker family of voltage-dependent K+ channels. The channel is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. It is in these cells (particularly T lymphocytes) that KV1.3 function has centered a lot of attention. It was found that KV1.3 is the main channel Responsible for maintaining the resting potential in quiescent cells and regulating the Ca2+ signaling that is indispensable for normal T lymphocyte activation.1,2 Based on the central role of Kv1.3 in regulating the initiation of an immune response, the channel has been recognized as a potential target for immunosuppressant drugs.1 KV1.3 channels are potently inhibited by several venomous peptide toxins among them Charybdotoxin (#STC-325, 2.6 nM), Noxiustoxin (#STN-340, 1 nM), Kaliotoxin (#STK-370, 0.65 nM), Margatoxin (#STM-325, 0.05 nM), Agitoxin-1 (#RTA-150, 1.7 nM), Agitoxin-2 (#RTA-420, 0.004 nM), Hongotoxin-1 (#RTH-400, 0.09 nM) and Stichodactyla toxin (#RTS-400, 0.01 nM).3-7
References 1. Chandy, K.G. et al. (2001) Toxicon 39, 1269. 2. Koo, G.C. et al. (1997) J. Immunol. 158, 5120. 3. Grissmer, S. et al. (1994) Mol. Pharmacol. 45, 1227. 4. Garcia-Calvo, M. et al. (1993) J. Biol. Chem. 268, 18866. 5. Garcia, M.L. et al. (1994) Biochemistry 33, 6834. 6. Koschak, A. et al. (1998) J. Biol. Chem. 273, 2639. 7. Kalman, K. et al. (1998) J. Biol. Chem. 273, 32697.
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