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Orexin Receptor 2 Antibody

Affinity purified polyclonal antibody

  • WB - Orexin Receptor 2 Antibody AG1157-050
    Western blot analysis of human Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) colon cancer cell lysates: 1, 2.  Anti-Orexin Receptor 2 antibody (#AG1157), (1:200).
    3, 4.  Anti-Orexin Receptor 2 antibody, preincubated with the control peptide antigen.
  • WB - Orexin Receptor 2 Antibody AG1157-050
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: 1, 2. Anti-Orexin Receptor 2 antibody (#AG1157), (1:400).
    3, 4. Anti-Orexin Receptor 2 antibody, preincubated with the control peptide antigen.
  • IHC - Orexin Receptor 2 Antibody AG1157-050
    Expression of OX2R in rat brain
    Immunohistochemical staining of rat brain using Anti-Orexin Receptor 2 antibody (#AG1157), (1:50), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (1:500), (red). A. In the cerebellum, staining is present in cell bodies of both Golgi type I (Purkinje cells – white arrows) and Golgi type II (green arrows) neurons. Staining is also present in fibers in the molecular layer (ML). B. In the parietal cortex, staining is evident in neural cell bodies. C. In the hippocampus, staining is present in pyramidal cells in the CA1 layer. Note that both cell bodies and prolongations are stained. Hoechst 33342 is used as the counterstain (blue).
  • ICC - Orexin Receptor 2 Antibody AG1157-050
    Expression of OX2R in human colon cancer cells Immunocytochemical staining of paraformaldehyde-fixed and permeabilized human Colo-205 colon cancer cells using Anti-Orexin Receptor 2 antibody (#AG1157), (1:500), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (red). B. Live view of the same field as in (A). C. Nuclei were visualized with the cell permeable dye Hoechst 33342 (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P56719
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 52489 Da
Homology Mouse - identical; human - 14/15 amino acid residues identical.
Additional Information
Gene ID 25605
Other Names Orexin receptor type 2, Ox-2-R, Ox2-R, Ox2R, Hypocretin receptor type 2, Hcrtr2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)DRLARGRTSTESRKS, corresponding to amino acid residues 391-405 of rat OX2R (Accession P56719). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Orexin Receptor 2 (OX2R) (also known as hypocretin receptor 2) is one of two receptors that recognize the peptide neurotransmitters orexin A and orexin B.1 Orexin A and B are 33 and 28 amino acids in length, respectively, and are derived from a common precursor termed prepro-orexin.   OX2R binds both orexin A and B with similar affinities while OX1R binds orexin A with greater affinity than orexin B (a one order of magnitude difference).2,3   Both OX2R and OX1R belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. OX2R is thought to transmit signals through the Ga11 class of G proteins, resulting in the activation of phospholipase C with subsequent triggering of the phosphatidylinositol cascade and an influx of extracellular Ca2+, probably through transient receptor potential (TRP) channels. OX2R can also transmit signals through inhibitory Gi G proteins, although the mechanism is less well understood.2,3   The physiological functions of the orexin system (OX1R, OX2R, and their ligands) have been a matter of intense research over the last few years. OX2R is expressed in both the central nervous system and peripheral locations such as gastrointestinal tissues, pancreas, and testis.2   The best studied physiological role of OX2R is its involvement in the regulation of sleep and wakefulness states. Studies in mice lacking the orexin gene and dogs expressing a null mutation of the OX2R show a remarkably similar phenotype to human narcolepsy, a condition characterized by excessive daytime sleepiness, inability to maintain vigilant states, and defects in the regulation of rapid eye movement (REM) sleep.4   In addition, the orexin system is involved in regulating autonomic functions such as blood pressure and heart rate, as well as in mechanisms that regulate the reward response in the brain.4   Abgent is pleased to offer a highly specific antibody directed against an epitope located in the intracellular C-terminal domain of rat Orexin Receptor 2. Anti-Orexin Receptor 2 antibody (#AG1157) can be used in western blot analysis, immunocytochemical and immunohistochemical applications, and recognizes OX2R from rat, mouse and human samples.


References 1. Sakurai, T. et al. (1998) Cell 92, 573. 2. Kukkonen, J.P. et al. (2002) Am. J. Physiol. Cell Physiol. 283, C1567. 3. Kirchgessner, A.L. (2002) Endocr. Rev. 23, 1 4. Sakurai, T. (2007) Nat. Rev. Neurosci. 8, 171.

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$ 475.00
$ 575.00
Cat# AG1157-050
(40 western blots)
Availability: 2-3 weeks
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