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>   home   >   Products   >   Primary Antibodies   >   Na+/H+ Exchanger 2 (NHE-2) Antibody   

Na+/H+ Exchanger 2 (NHE-2) Antibody

Affinity purified polyclonal antibody

  • WB - Na+/H+ Exchanger 2 (NHE-2) Antibody AG1161-050
    Western blot analysis of rat kidney membranes (lanes 1 and 4), mouse kidney lysate (lanes 2 and 5) and rat stomach lysate (lanes 3 and 6): 1-3. Anti-Na+/H+ Exchanger 2 (NHE-2) antibody (#AG1161), (1:200).
    4-6. Anti-Na+/H+ Exchanger 2 (NHE-2) antibody, preincubated with the control peptide antigen.
  • WB - Na+/H+ Exchanger 2 (NHE-2) Antibody AG1161-050
    Western blot analysis of human Colo-205 colorectal carcinoma cell lysates: Western blot analysis of human Colo-205 colorectal carcinoma cell lysates:
    1. Anti-Na+/H+ Exchanger 2 (NHE-2) antibody (#AG1161), (1:200).
    2. Anti-Na+/H+ Exchanger 2 (NHE-2) antibody, preincubated with the control peptide antigen.
  • IHC - Na+/H+ Exchanger 2 (NHE-2) Antibody AG1161-050
    Expression of Na+/H+ Exchanger 2 (NHE-2) in rat colon Immunohistochemical staining of rat colon sections (paraffin-embedded) using Anti-Na+/H+ Exchanger 2 (NHE-2) antibody (#AG1161), (1:50). A. NHE-2 staining is shown in red. B. Cell nuclei were labeled with DAPI (blue). C. Merge of the two images.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P48763
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 91403 Da
Homology Mouse - identical; human - 12/16 amino acid residues identical.
Additional Information
Gene ID 24783
Other Names Sodium/hydrogen exchanger 2, H7, Na(+)/H(+) exchanger 2, NHE-2, Solute carrier family 9 member 2, Slc9a2, Nhe2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)RASEPGNRKGRLGNEK, corresponding to amino acid residues 797-812 of rat NHE-2 (Accession P48763). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
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In order to function in optimal conditions, cells must maintain a close to neutral intracellular pH. They have adopted various mechanisms in order to do so, one of which is via Na+/H+ exchangers (NHEs). Genes belonging to this group are expressed along a very broad range of organisms and are essential for protecting cells against intracellular acidification1. To date, nine genes have been identified in mammals; NHE1-9. These membrane proteins have 10-12 transmembrane domains depending on whether a splice variant is expressed and an intracellular N-terminal. The C-terminal domain can be either intracellular or extracellular, also depending whether a splice variant of the protein is involved. The C-terminal part of the protein also undergoes posttranslational modification such as phosphorylation2. Both NHE-1 and NHE-2 have an extracellular loop which is glycosylated1,3,4. Under physiological conditions, the Na+/H+ exchanger mediates the exchange of one extracellular Na+ ion for one intracellular proton, thereby keeping the overall charge neutral1. The extracellular binding site of Na+ is not selective as it can also bind Li+ and H+ 1,5,6.  K+ ions inhibit NHE-1 but have no effect on NHE-27. The activation of NHE-1 and NHE-2 is sensitive to intracellular acidic pH. Under physiological conditions, both exchangers are not active and upon a drop of intracellular pH, they are rapidly activated1,5,8. NHE-2 is detected in the intestine, kidney and parietal cells2,9-11. It is also detected in skeletal muscle and testis2,12. Abgent is pleased to offer a highly specific antibody directed against an epitope of rat NHE-2. Anti-Na+/H+ Exchanger 2 (NHE-2) antibody (#AG1161) can be used in western blot analysis and immunohistochemistry applications, and has been designed to recognize NHE-2 from human, rat and mouse samples.


References 1. Counillon, L. and Pouyssegur, J. (2000) J. Biol. Chem. 275, 1. 2. Kemp, G. et al. (2008) Channels 2, 329. 3. Counillon, L. et al. (1994) Biochemistry 33, 10463. 4. Tse, C. et al. (1994) Biochemistry 33, 12954. 5. Paris, S. and Poouyssegur, J. (1983) J. Biol. Chem. 258, 3503. 6. Aronson, P.S. et al. (1983) J. Biol. Chem. 258, 6767. 7. Yu, F.H. et al. (1993) J. Biol. Chem. 268, 25536. 8. Aronson, P.S. et al. (1982) Nature 299, 161. 9. Bookstein, C. et al. (1997) Am. J. Physiol. 273, 1496. 10. Chambrey, R. et al. (1998) Am. J. Physiol. 275, 379. 11. Schultheis, P.J. et al. (1998) Nat. Genet. 19, 282. 12. Malakooti, J. et al. (1999) Am. J. Physiol. 277, 383.

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$ 367.00
$ 475.00
Cat# AG1161-050
Availability: 2-3 weeks
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