|Calculated MW||71001 Da|
|Homology||Mouse - 13/15 amino acid residues identical; human- 12/15 amino acid residues identical.|
|Other Names||Sodium/nucleoside cotransporter 1, Concentrative nucleoside transporter 1, CNT 1, Na(+)/nucleoside cotransporter 1, Sodium-coupled nucleoside transporter 1, Solute carrier family 28 member 1, Slc28a1, Cnt1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)ENMGAEFLESMEEGR, corresponding to amino acid residues 22-36 of rat Concentrative Nucleoside Transporter 1 (Accession Q62674). Intracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Nucleosides play other important roles beyond their nucleic acid synthesis building block role. For example, they are involved in energy metabolism; they serve as ligands of purinergic receptors and act as influential signaling molecules1. Being hydrophilic, nucleosides cannot simply diffuse across the plasma membrane in order to exert their various functions, but rather need to be physically transported via nucleoside transporters1,2. Two different transporter families are responsible for transporting nucleosides across the plasma membrane: The Concentrative Nucleoside Transporter proteins (CNT, SLC28 family), which consist of three members, CNT1-3, and act as Na+-dependent symporters1,3. The Equilibrative Nucleoside Transporter proteins ENT1-4 (ENT, SLC29 family), which mediate a Na+-independent facilitated diffusion. Therefore, ENTs act as bidirectional carriers, responsible for the influx and efflux of substrates1. CNTs are responsible for the intracellular uptake of nucleosides, an energy consuming process which is coupled to the Na+ gradient across the plasma membrane. All three transporters display a relatively high affinity for their substrates but a more selective than ENTs1,4. Pyrimidines are the substrate of choice for CNT1 although it can bind but not transport adenosine. CNT2 prefers purines, although it can transport uridine, and CNT3 is selective for both types of nucleosides. Both CNT1 and CNT2 display a 1:1 stoichiometry (nucleoside:sodium)1,5. CNT3 on the other hand transports two Na+ ions per nucleoside, and can also co-transport protons in a pH dependent manner in a 1:1 ratio1,6,7. All three CNTs have thirteen transmembrane domains and an extracellular C-terminus responsible for substrate recognition1. CNT1 is widely expressed in the small intestine, kidney and liver1,8-10. CNT2 is expressed in immune system cells1,8 and CNT3 expression is abundant in monocytes and macrophages. All three are detected in various regions of the brain1,6,11,12. There is no evidence that nucleoside transporters are directly involved in pathophysiologies, but they are clinically significant. For example, nucleoside transporters are responsible for the cellular uptake of a number of nucleoside-derived anticancer drugs1. Abgent is pleased to offer a highly specific antibody directed against an epitope of rat CNT1. Anti-Concentrative Nucleoside Transporter 1 (CNT1) antibody (#AG1163) can be used in western blot analysis and was designed to recognize CNT1 from human, rat and mouse samples.
References 1. Molina-Arcas, M. et al. (2009) Curr. Vasc. Pharmacol. 7, 426. 2. Rose, J.B. and Coe, I.R. (2008) Physiology 23, 41. 3. Baldwin, S.A. et al. (2004) Pflugers Arch. 447, 735. 4. Gray, J.H. et al. (2004) Pflugers Arch. 447, 728. 5. Ritzel, M.W. et al. (1998) Mol. Membr. Biol. 15, 203. 6. Ritzel, M.W. et al. (2001) J. Biol. Chem. 276, 2914. 7. Smith, K.M. et al. (2005) J. Biol. Chem. 280, 25436. 8. Pennycooke, M. et al. (2001) Biochem. Biophys. Res. Commun. 280, 951. 9. Felipe, A. et al. (1998) Biochem. J. 330 (Pt.2), 997. 10. Valdes, R. et al. (2000) Gastroenterology 119, 1623. 11. Anderson, C.M. et al. (1996) Brain Res. Mol. Brain Res. 42, 358. 12. Guillen-Gomez, E. et al. (2004) J. Neurochem. 90, 883.
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