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CNTF Antibody

Affinity purified polyclonal antibody

  • WB - CNTF Antibody AG1166-050
    Western blot analysis using Anti-CNTF on:
    1. human CNTF (#C-240), 20ng.
    2. hCardiotrophin-1 (#C-200), 20ng.
    3. hLIF (#L-200), 20ng.
    4. mIL-6 (#I-220), 20 ng.
    5. human CNTF (#C-240), 20ng.
    Lanes 1-4.  Anti-CNTF antibody (#AG1166), (1:200).
    Lane 5.  Anti-CNTF, preincubated with the control peptide antigen.
    The specificity of the antibody is demonstrated in that it specifically recognizes CNTF while it does not reacts with the related cytokines Cardiotrophin-1, LIF and IL-6.
  • IHC - CNTF Antibody AG1166-050
    Expression of Ciliary Neurotrophic Factor (CNTF) in rat hippoccampus Immunohistochemical staining of rat hippoccampus using Anti-CNTF antibody (#AG1166), (1:200). A. CNTF staining (green) reveals astrocyte-profiles (arrows show examples) in the hilus (H) of the dentate gyrus. B. Staining the same section with the astrocyte marker, anti-glial fibrillary acidic protein (red), visualizes astrocytes in the hilus. C. Merge of the two images shows that CNTF is indeed expressed in astrocytes.
  • ICC - CNTF Antibody AG1166-050
    Expression of CNTF in rat C6 glioma cells Immunocytochemical staining of fixed and permeabilized rat C6 glioma cells. A. Cells were stained with Anti-CNTF antibody (#AG1166), (1:200), followed by goat anti-rabbit-DyLight-594 secondary antibody (red). B. Cell nuclei were visualized with the membrane-permeable DNA dye Hoechst 33342 (blue staining). C. Live view of the cells.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P26441
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 22931 Da
Homology Rat, mouse- identical.
Additional Information
Gene ID 1270
Other Names Ciliary neurotrophic factor, CNTF, CNTF
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)ELSQWTVRSIHDLR, corresponding to amino acid residues 164-177 of human CNTF (Accession P26441). C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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CNTF is a polypeptide trophic factor, member of the alpha-helical cytokine superfamily1. It was initially purified from the chick eye on the basis of its ability to promote survival of E8 chick ciliary ganglion neurons in culture2. CNTF is synthesized by glia both in the CNS and PNS3 and it has been demonstrated that CNTF is ubiquitously distributed in neurons and glia throughout the rodent brain4. CNTF effects are mediated by a tripartite receptor complex consisting of two signal-transducing subunits (leukemia inhibitory factor receptor, gp130) and a CNTF-specific ligand-binding-subunit (CNTFR)5.  CNTF can support the survival of many different cell populations within the PNS and the CNS6. in vitro, CNTF promotes proliferation and neuronal specifications in hippocampal neurons. in vivo, it supports the viability of non-primate motor neurons7, CNTF induces sprouting of cholinergic motor neurons8 and delays neural degeneration in genetic models of motor neuron disease9. In addition, it is involved in the development stage of astrocytes and oligodendocytes10. Abgent is pleased to offer a highly specific antibody directed against an epitope of human CNTF. Anti-CNTF (#AG1166) antibody can be used in western blot and immunocytochemical applications, and has been designed to recognize CNTF from human, rat and mouse samples. In addition, Anti-CNTF specifically recognizes CNTF but not related cytokines.


References 1. Mufson, E.J. et al. (1999) Prog. Neurobiol. 57, 451. 2. Adler, R. et al. (1979) Science 204, 1434. 3. Murphy, M. et al. (1997) Prog. Neurobiol. 52, 355 4. Henderson, J.T. et al. (1994) Mol. Brain Res. 22, 151. 5. Stahl, N. et al. (1993) J. Biol. Chem. 268, 7628. 6. Richardson, P.M. (1994) Pharmacol. Ther. 63, 187. 7. Ip, N.Y. et al. (1991) J. Physiol. 85, 123. 8. Gurney, M.E. et al. (1992) J. Neurosci. 12, 3241. 9. Sendtner, M. et al. (1992) Nature 358,502. 10. Mayer, M. et al. (1994) Development 120, 143.

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$ 475.00
$ 575.00
Cat# AG1166-050
(40 western blots)
Availability: 2-3 weeks
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