|Calculated MW||22931 Da|
|Homology||Rat, mouse- identical.|
|Other Names||Ciliary neurotrophic factor, CNTF, CNTF|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)ELSQWTVRSIHDLR, corresponding to amino acid residues 164-177 of human CNTF (Accession P26441). C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
CNTF is a polypeptide trophic factor, member of the alpha-helical cytokine superfamily1. It was initially purified from the chick eye on the basis of its ability to promote survival of E8 chick ciliary ganglion neurons in culture2. CNTF is synthesized by glia both in the CNS and PNS3 and it has been demonstrated that CNTF is ubiquitously distributed in neurons and glia throughout the rodent brain4. CNTF effects are mediated by a tripartite receptor complex consisting of two signal-transducing subunits (leukemia inhibitory factor receptor, gp130) and a CNTF-specific ligand-binding-subunit (CNTFR)5. CNTF can support the survival of many different cell populations within the PNS and the CNS6. in vitro, CNTF promotes proliferation and neuronal specifications in hippocampal neurons. in vivo, it supports the viability of non-primate motor neurons7, CNTF induces sprouting of cholinergic motor neurons8 and delays neural degeneration in genetic models of motor neuron disease9. In addition, it is involved in the development stage of astrocytes and oligodendocytes10. Abgent is pleased to offer a highly specific antibody directed against an epitope of human CNTF. Anti-CNTF (#AG1166) antibody can be used in western blot and immunocytochemical applications, and has been designed to recognize CNTF from human, rat and mouse samples. In addition, Anti-CNTF specifically recognizes CNTF but not related cytokines.
References 1. Mufson, E.J. et al. (1999) Prog. Neurobiol. 57, 451. 2. Adler, R. et al. (1979) Science 204, 1434. 3. Murphy, M. et al. (1997) Prog. Neurobiol. 52, 355 4. Henderson, J.T. et al. (1994) Mol. Brain Res. 22, 151. 5. Stahl, N. et al. (1993) J. Biol. Chem. 268, 7628. 6. Richardson, P.M. (1994) Pharmacol. Ther. 63, 187. 7. Ip, N.Y. et al. (1991) J. Physiol. 85, 123. 8. Gurney, M.E. et al. (1992) J. Neurosci. 12, 3241. 9. Sendtner, M. et al. (1992) Nature 358,502. 10. Mayer, M. et al. (1994) Development 120, 143.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.