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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Ret (extracellular) Antibody   

Ret (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - Ret (extracellular) Antibody AG1168-025
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
    1,2. Anti-Ret (extracellular) antibody (#AG1168), (1:200).
    3,4. Anti-Ret (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Ret (extracellular) Antibody AG1168-025
    Expression of Ret in rat DRG Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-Ret (extracellular) antibody (#AG1168), (1:50). Ret staining (red) is detected in DRG neurons. Hoechst 33342 (blue) is used to stain cell nuclei.
  • ICC - Ret (extracellular) Antibody AG1168-025
    Expression of Ret in human MCF-7 breast adenocarcinoma cells Immunocytochemical staining of live intact human MCF-7 breast adenocarcinoma cells. A. Extracellular staining of cells with Anti-Ret (extracellular) antibody (#AG1168), (1:25), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of the two images.
  • FC - Ret (extracellular) Antibody AG1168-025
    Indirect flow cytometry analysis of live intact human THP-1 monocytic leukemia cell line:
    ___ Unstained cells + goat-anti-rabbit-AlexaFluor-647 secondary antibody.
    ___ Cells + Anti-Ret (extracellular) antibody (#AG1168), (1:20) + goat-anti-rabbit-AlexaFluor-647 secondary antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P07949
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 124319 Da
Homology Mouse - identical; rat - 12/13 amino acid residues identical.
Additional Information
Gene ID 5979
Other Names Proto-oncogene tyrosine-protein kinase receptor Ret, Cadherin family member 12, Proto-oncogene c-Ret, Soluble RET kinase fragment, Extracellular cell-membrane anchored RET cadherin 120 kDa fragment, RET, CDHF12, CDHR16, PTC, RET51
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CEWRQGDGKGITR, corresponding to amino acid residues 541-553 of human Ret (Accession P07949). Extracellular, N-terminus.This antibody will recognize the intact Ret receptor and the cleaved Ret extracellular cell-membrane anchored fragment. It won't recognize the cleaved Ret kinase fragment.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Resuspended antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
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The RET proto-oncogene on human chromosome 10q11.2, encodes a receptor tyrosine kinase (RTK) activated by members of the glial cell line-derived neurotrophic factor (GDNF) ligand family (GDNF, neurturin, artemin, and persephin) in conjunction with a ligand-specific coreceptor (GFRα1-4)1. RET belongs to the cadherin superfamily, and it has been suggested that the ret gene is the result of a recombination between a gene encoding a tyrosine kinase receptor and a gene encoding an ancestral cadherin at an early stage of evolution2. The extracellular domain of RET comprises, as observed for other tyrosine kinase receptors, a cysteine-rich domain, but also four cadherin-like domains3.   RET signaling is crucial for the development of the enteric nervous system. RET also regulates the development of sympathetic, parasympathetic, motor, and sensory neurons, and is necessary for the postnatal maintenance of dopaminergic neurons. Outside the nervous system, RET is crucial for development of the kidney and plays a key role in spermatogenesis3.   RET has attracted considerable clinical interest because of the range of mutations found in diverse conditions that include Hirschsprung disease and a variety of cancers involving the thyroid gland. RET-related cancers with thyroid involvement include sporadic and familial medullary thyroid carcinoma (MTC), multiple endocrine neoplasia 2 (MEN2) syndromes MEN2A and MEN2B, and papillary thyroid carcinoma (PTC)4


References 1. Durbec, P. et al. (1996) Nature 381, 789. 2. Cabrera J.R. et al. (2011) J. Biol. Chem. 286, 14628. 3. Anders, J. et al. (2001) J. Biol. Chem. 276, 35808. 4. Runeberg-Roos, P. and Saarma, M. (2007) Ann. Med. 39, 572. 5. Knowles, P.P. et al. (2006) J. Biol. Chem. 281, 33577.

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$ 247.00
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Cat# AG1168-025
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