|Application ||WB, LCI|
|Calculated MW||29682 Da|
|Homology||Rat - 12/13 amino acid residues identical; mouse - 11/13 amino acid residues identical.|
|Other Names||GDNF family receptor alpha-4, GDNF receptor alpha-4, GDNFR-alpha-4, GFR-alpha-4, Persephin receptor, Gfra4|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)GSRDG(S)PEEGGPR, corresponding to amino acid residues 160-172 of rat GFR־±4 (Accession Q9EPI2). Extracellular, N-terminus.Not recommended for human samples. The antibody will recognize both the membrane bound canonical isoform and the putative secreted isoform.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||2 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The GDNF family ligands (GFLs) belong to the super family of the TGF-β. They belong to the group of cystine-knot protein and function as homodimers1. This family includes glial cell line-derived neurotrophic factor (GDNF), artemin (ARTN), Neurturin (NRTN) and persephin (PSPN)2. These factors are heavily involved in the development and function of the nervous system (both central and peripheral). In particular GDNF has an important role outside the nervous system where it plays a role in kidney morphogenesis1. In general GFLs all signal through a signal through the receptor tyrosine kinase Ret. Their specificity is implemented by different GDNF family receptor a (GFRα), which act as co-receptors. These extracellular proteins are bound to the plasma membrane via a glycosyl phosphatidyl inositol (GPI) anchor. GFRα1-4 are responsible for the binding of GDNF, NRTN, ARTN, and PSPN respectively and the subsequent activation of Ret. Soluble forms of the receptor by the cleavage of a yet unknown phospholipase or protease can be detected1,3. Also alternative spliced forms of the protein can lead to soluble forms of GFRα receptors3.
References 1. Airaksinen, M.S. and Saarma, M. (2002) Nat. Rev. Neurosci. 3, 383. 2. Paratcha, G. and Ledola, F. (2008) Trends Neurosci. 31, 384. 3. Paratcha, G. et al. (2001) Neuron 29, 171.
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