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>   home   >   Products   >   Primary Antibodies   >   GDNF Family Receptor Alpha4 (extracellular) Antibody   

GDNF Family Receptor Alpha4 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - GDNF Family Receptor Alpha4 (extracellular) Antibody AG1169-050
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: 1-2. Anti-GDNF Family Receptor α4 (extracellular) antibody (#AG1169), (1:800).
    3-4. Anti-GDNF Family Receptor α4 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - GDNF Family Receptor Alpha4 (extracellular) Antibody AG1169-050
    Expression of GFRα4 in rat red nucleus Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GDNF Family Receptor α4 (extracellular) antibody (#AG1169), (1:100). Staining reveals expression of GFRα4 (red) in cells with neuronal outline (arrows point at some examples) in the red nucleus. DAPI is used as the counterstain (blue).
  • ICC - GDNF Family Receptor Alpha4 (extracellular) Antibody AG1169-050
    Expression of GFRα4 in rat PC12 cells Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. Extracellular staining of cells with Anti-GDNF Family Receptor α4 (extracellular) antibody (#AG1169), (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei stained with the cell permeable dye Hoechst 33342 (blue). C. Merge of the two images.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9EPI2
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 29682 Da
Homology Rat - 12/13 amino acid residues identical; mouse - 11/13 amino acid residues identical.
Additional Information
Gene ID 66023
Other Names GDNF family receptor alpha-4, GDNF receptor alpha-4, GDNFR-alpha-4, GFR-alpha-4, Persephin receptor, Gfra4
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)GSRDG(S)PEEGGPR, corresponding to amino acid residues 160-172 of rat GFR־±4 (Accession Q9EPI2). Extracellular, N-terminus.Not recommended for human samples. The antibody will recognize both the membrane bound canonical isoform and the putative secreted isoform.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 2 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
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The GDNF family ligands (GFLs) belong to the super family of the TGF-β. They belong to the group of cystine-knot protein and function as homodimers1. This family includes glial cell line-derived neurotrophic factor (GDNF), artemin (ARTN), Neurturin (NRTN) and persephin (PSPN)2. These factors are heavily involved in the development and function of the nervous system (both central and peripheral). In particular GDNF has an important role outside the nervous system where it plays a role in kidney morphogenesis1. In general GFLs all signal through a signal through the receptor tyrosine kinase Ret. Their specificity is implemented by different GDNF family receptor a (GFRα), which act as co-receptors. These extracellular proteins are bound to the plasma membrane via a glycosyl phosphatidyl inositol (GPI) anchor. GFRα1-4 are responsible for the binding of GDNF, NRTN, ARTN, and PSPN respectively and the subsequent activation of Ret. Soluble forms of the receptor by the cleavage of a yet unknown phospholipase or protease can be detected1,3. Also alternative spliced forms of the protein can lead to soluble forms of GFRα receptors3.


References 1. Airaksinen, M.S. and Saarma, M. (2002) Nat. Rev. Neurosci. 3, 383. 2. Paratcha, G. and Ledola, F. (2008) Trends Neurosci. 31, 384. 3. Paratcha, G. et al. (2001) Neuron 29, 171.

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$ 475.00
$ 575.00
Cat# AG1169-050
(40 western blots)
Availability: 2-3 weeks
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