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>   home   >   Products   >   Primary Antibodies   >   GDNF Family Receptor alpha1 (extracellular) Antibody   

GDNF Family Receptor alpha1 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - GDNF Family Receptor alpha1 (extracellular) Antibody AG1170-050
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1, 2. Anti-GDNF Family Receptor α1 (extracellular) antibody (#AG1170), (1:200).
    3, 4. Anti-GDNF Family Receptor α1 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - GDNF Family Receptor alpha1 (extracellular) Antibody AG1170-050
    Expression of GFRα1 in rat neocortex Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GDNF Family Receptor α1 (extracellular) antibody (#AG1170), (1:100). A. GFRα1 (green) is visualized in neocortex neurons. B. Neurons expressing γ-amino butyric acid (GABA) are labeled with parvalbumin (red). C, Merge of the two images demonstrates partial colocalization (arrows).
  • ICC - GDNF Family Receptor alpha1 (extracellular) Antibody AG1170-050
    Expression of GFRα1 in live intact rat C6 glioma cells Immunocytochemical staining of live intact rat C6 glioma cells with Anti-GDNF Family Receptor α1 (extracellular) antibody (#AG1170), (1:50), followed by goat-anti-rabbit-DyLight-594 secondary antibody (red) (A). B. Live view of the cells. C. Merge of the two images.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q62997
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 51650 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 25454
Other Names GDNF family receptor alpha-1, GDNF receptor alpha-1, GDNFR-alpha-1, GFR-alpha-1, RET ligand 1, TGF-beta-related neurotrophic factor receptor 1, Gfra1, Gdnfra, Retl1, Trnr1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CRSAMEALKQKS, corresponding to amino acid residues 72-83 of rat GFR־±1 (Accession Q62997). Extracellular domain.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Resuspended antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
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The GDNF family ligands (GFLs) belong to the super family of the TGF-β. They belong to the group of cystine-knot protein and function as homodimers1. This family includes glial cell line-derived neurotrophic factor (GDNF), artemin (ARTN), Neurturin (NRTN) and persephin (PSPN)2. These factors are heavily involved in the development and function of the nervous system (both central and peripheral). In particular GDNF has an important role outside the nervous system where it plays a role in kidney morphogenesis1. In general GFLs all signal through a signal through the receptor tyrosine kinase Ret. Their specificity is implemented by different GDNF family receptor a (GFRα), which act as co-receptors. These extracellular proteins are bound to the plasma membrane via a glycosyl phosphatidyl inositol (GPI) anchor. GFRα1-4 are responsible for the binding of GDNF, NRTN, ARTN, and PSPN respectively and the subsequent activation of Ret. Soluble forms of the receptor by the cleavage of a yet unknown phospholipase or protease can be detected1,3. Also alternative spliced forms of the protein can lead to soluble forms of GFRα receptors3. In respect to their important role in development, individual knockout of either gdnf, gfra1 or ret gives rise to lethal phenotypes2.


References 1. Airaksinen, M.S. and Saarma, M. (2002) Nat. Rev. Neurosci. 3, 383. 2. Paratcha, G. and Ledola, F. (2008) Trends Neurosci. 31, 384. 3. Paratcha, G. et al. (2001) Neuron 29, 171.

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$ 475.00
$ 575.00
Cat# AG1170-050
(40 western blots)
Availability: 2-3 weeks
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