|Calculated MW||46266 Da|
|Homology||Mouse - 14/16 amino acid residues identical.|
|Other Names||Neurotensin receptor type 2, NT-R-2, NTR2, High-affinity levocabastine-sensitive neurotensin receptor, Ntsr2, Ntr2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CGEQHSLVPLPQEAPE, corresponding to amino acidresidues 377-392 of rat Neurotensin Receptor 2 (Accession Q63384 ). Intracellular, C-terminus.Unlikely to recognize human samples.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Neurotensin receptor 2 (NTS2) is one of three receptors that mediate the effects of the tridecapeptide neurotensin. Neurotensin is synthesized and secreted from neurons in the central neural system (CNS) and from endocrine cells in the gastrointestinal tract. NTS2 and NTS1 belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily while the third neurotensin receptor NTS3 (also called Sortilin) is a type I membrane protein with a large extracellular domain. Both NTS1 and NTS3 bind neurotensin with high affinity while NTS2 binds it with low affinity.1 NTS2 signals preferentially through Gaq, resulting in the activation phopholipase C and intracellular Ca2+ mobilization. However, the exact signaling mechanisms subsequent to NTS2 activation appear to depend on the species and on the cellular system in which the receptor is studied. NTS2 is expressed mainly in the central nervous system and dorsal root ganglion (DRG) neurons but has been also observed in peripheral tissues such as gastric parietal cells where it probably mediates the neurotensin-induced effects on gastric acid secretion.2 The most established physiological role of NTS2 is its role in pain transmission as NTS2-defficient mice showed that NTS2 is the likely mediator of the neurotensin-induced analgesic responses.3 Abgent is pleased to offer a highly specific antibody directed against an epitope at the intracellular C-terminal domain of the rat NTS2. Anti-Neurotensin Receptor 2 antibody (#AG1173) can be used in Western blot analysis, immunohistochemical and immunocytochemical applications, and recognizes NTS2 receptor from rat and mouse samples.
References 1. Vincent, J.P. et al. (1999) Trends Pharmacol. Sci. 20, 302. 2. Schulz, S. et al. (2006) J. Endocrinol. 191, 121. 3. Maeno, H. et al. (2004) Brain Res. 998, 122.
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