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Neurotensin Receptor 2 Antibody

Affinity purified polyclonal antibody

     
  • WB - Neurotensin Receptor 2 Antibody AG1173-050
    Western blot analysis of rat dorsal root ganglion lysates: 1. Anti-Neurotensin Receptor 2 antibody (#AG1173), (1:200).
    2. Anti-Neurotensin Receptor 2 antibody, preincubated with the control peptide antigen.
  • WB - Neurotensin Receptor 2 Antibody AG1173-050
    Western blot analysis of rat brain lysates: 1. Anti-Neurotensin Receptor 2 antibody (#AG1173), (1:200).
    2. Anti-Neurotensin Receptor 2 antibody, preincubated with the control peptide antigen.
  • WB - Neurotensin Receptor 2 Antibody AG1173-050
    Western blot analysis of mouse brain lysates: 1. Anti-Neurotensin Receptor 2 antibody (#AG1173), (1:400).
    2. Anti-Neurotensin Receptor 2 antibody, preincubated with the control peptide antigen.
  • IHC - Neurotensin Receptor 2 Antibody AG1173-050
    Expression of NTR2 in rat mediobasal hypothalamus Immunohistochemical staining of perfusion-fixed brain frozen rat mediobasal (MB) hypothalamus sections using Anti-Neurotensin Receptor 2 antibody (#AG1173), (1:100), (green). A. Neurotensin Receptor 2 (NTS2) appears in neuronal fibers marked by white arrows. B. In the same section, there is staining for glial fibrillary acidic protein (red fluorescence), a marker of astrocytes. C. Merging the two images demonstrates that NTS2 is not localized in astrocytes. DAPI is used as the counterstain (blue).
  • IHC - Neurotensin Receptor 2 Antibody AG1173-050
    Expression of NTR2 in rat stomach Immunohistochemical staining of paraffin-embedded rat stomach sections using Anti-Neurotensin Receptor 2 antibody (#AG1173), (1:50), (brown). Staining is specific for parietal cells (arrows) of the gastric glands. Hematoxilin is used as the counterstain.
  • ICC - Neurotensin Receptor 2 Antibody AG1173-050
    Expression of NTR2 in rat C6 glioma cells Immunocytochemical staining of rat C6 glioma cells. A. Paraformaldehyde fixed and permeabilized rat C6 glioma cells were stained with Anti-Neurotensin Receptor 2 antibody (#AG1173), (1:200), followed by Alexa-555 conjugated goat-anti-rabbit- secondary antibody (orange staining). Nuclei were visualized with the cell permeable dye Hoechst 33342 (blue staining). B. Live view of the same field as in (A).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q63384
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 46266 Da
Homology Mouse - 14/16 amino acid residues identical.
Additional Information
Gene ID 4804
Other Names Neurotensin receptor type 2, NT-R-2, NTR2, High-affinity levocabastine-sensitive neurotensin receptor, Ntsr2, Ntr2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CGEQHSLVPLPQEAPE, corresponding to amino acidresidues 377-392 of rat Neurotensin Receptor 2 (Accession Q63384 ). Intracellular, C-terminus.Unlikely to recognize human samples.
Dilution WB~~1:200-1:2000
IHC~~1:50
ICC~~1:200
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Background

Neurotensin receptor 2 (NTS2) is one of three receptors that mediate the effects of the tridecapeptide neurotensin. Neurotensin is synthesized and secreted from neurons in the central neural system (CNS) and from endocrine cells in the gastrointestinal tract.   NTS2 and NTS1 belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily while the third neurotensin receptor NTS3 (also called Sortilin) is a type I membrane protein with a large extracellular domain. Both NTS1 and NTS3 bind neurotensin with high affinity while NTS2 binds it with low affinity.1   NTS2 signals preferentially through Gaq, resulting in the activation phopholipase C and intracellular Ca2+ mobilization. However, the exact signaling mechanisms subsequent to NTS2 activation appear to depend on the species and on the cellular system in which the receptor is studied.   NTS2 is expressed mainly in the central nervous system and dorsal root ganglion (DRG) neurons but has been also observed in peripheral tissues such as gastric parietal cells where it probably mediates the neurotensin-induced effects on gastric acid secretion.2   The most established physiological role of NTS2 is its role in pain transmission as NTS2-defficient mice showed that NTS2 is the likely mediator of the neurotensin-induced analgesic responses.3   Abgent is pleased to offer a highly specific antibody directed against an epitope at the intracellular C-terminal domain of the rat NTS2. Anti-Neurotensin Receptor 2 antibody (#AG1173) can be used in Western blot analysis, immunohistochemical and immunocytochemical applications, and recognizes NTS2 receptor from rat and mouse samples.

References

References 1. Vincent, J.P. et al. (1999) Trends Pharmacol. Sci. 20, 302. 2. Schulz, S. et al. (2006) J. Endocrinol.  191, 121. 3. Maeno, H. et al. (2004) Brain Res. 998, 122.

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Cat# AG1173-050
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