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GDNF Antibody

Affinity purified polyclonal antibody

  • WB - GDNF Antibody AG1175-050
    Western blot analysis of recombinant human GDNF (hGDNF):
    Lanes 1, 4:  50 ng hGDNF (#G-240).
    Lanes 2, 5:  10 ng hGDNF.
    Lanes 3, 4 ng hGDNF.
    Lanes 1-3: Anti-GDNF antibody (#AG1175), (1:1000).
    Lanes 4-5: Anti-GDNF antibody preincubated with the control peptide antigen.
  • IHC - GDNF Antibody AG1175-050
    Expression of GDNF in rat dorsal root ganglion (DRG).
    Immunohistochemical staining of rat DRG longitudinal frozen section using Anti-GDNF antibody (#AG1175), (1:50), followed by Alexa 555-conjugated goat anti-rabbit antibody (red staining). Hoechst 33342 (blue) was used as a counterstain. GDNF staining is specific for neurons. Note that Schwann cells as well as satellite glial cells are both negative.
  • IHC - GDNF Antibody AG1175-050
    Expression of GDNF in rat hippocampus Immunohistochemical staining of rat hippocampus frozen sections with Anti-GDNF antibody (#AG1175), (1:100), (red). A. GDNF staining is apparent in the pyramidal layer (asterik), in astrocytic fibers (horizontal arrows) and in some interneurons (vertical arrow). B. Calbindin D28k (green) appears in a subset of cells in the pyramidal layer. C. Merge of GDNF and calbindin demonstrates some co-localization in neurons. DAPI is used as the counterstain (blue).
  • ICC - GDNF Antibody AG1175-050
    Expression of GDNF in rat C6 glioma cells Immunocytochemical staining of GDNF in rat C6 glioma cells. A. Paraformaldehyde-fixed and permeabilized rat C6 glioma cells were stained with Anti-GDNF antibody (#AG1175), (1:200) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). Nuclei were visualized with the cell-permeable dye Hoechst 33342 (blue). B. Live view of the same field as in (A).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P39905
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 23720 Da
Homology Mouse, dog, monkey, bovine- identical; rat 15/16 amino acid residuesidentical.
Additional Information
Gene ID 2668
Other Names Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)DDNLVYHILRKHSAKR, corresponding to amino acid residues 192-207 of human GDNF (precursor) (Accession P39905). C-terminus of the mature GDNF.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Glial cell-line derived neurotrophic factor (GDNF) is a neurotrophic factor originally identified for its ability to promote the survival of dopaminergic neurons in the midbrain.1 GDNF is part of the neurotrophic factor family known as GDNF-family ligand (GFL), which includes neurturin (NRTN), artemin (ARTN), and persephin (PSPN).2,3 The GFL ligands are structurally related to the transforming growth factor-b (TGF-b) protein superfamily and contain seven cysteine residues with the same relative spacing as other members of this family.2 Typical of neurotrophic factors, GDNF is produced in the form of a precursor, preproGDNF, which is then cleaved during secretion to become proGDNF, and later processed to the mature (homodimeric) GDNF form.2 The GFLs convey their activity by binding to a multicomponent receptor that includes the RET receptor tyrosine kinase and a glycosyl phosphatidylinositol (GPI)-linked ligand-binding subunit known as GDNF family receptor a (GFRa).2,3 Four different GFRa subunits have been identified (GFRa 1-4) that determine the specificity of the GFRa-RET receptor complex. Thus, GFRa1 together with RET make up the high affinity receptor for GDNF. The GDNF and GFRa complex interaction with RET induces activation of the intracellular tyrosine kinase domain of the latter. Phosphorylated RET can then interact with several intracellular signaling cascades including MAP kinase, PI3K and PLCg signaling pathways.2,3   Lately a RET-independent signaling pathway for GDNF has been shown to involve binding of GDNF and GFRa to the neural cell adhesion molecule (NCAM). Signaling of GDNF via the NCAM complex stimulates intracellular tyrosine kinases such as Fyn and FAK.4   GDNF is synthesized and secreted by a wide variety of cell types in the central and peripheral nervous system where it exerts its neurotrophic effects in the development and maintenance of distinct sets of neurons. Accordingly, GDNF knockout mice die shortly after birth, reflecting the central role of GDNF in neurodevelopment.   Remarkably, GDNF was found to be an essential morphogen for kidney formation as well as to have a role is spermatogenesis.3   Abgent is pleased to offer a highly specific antibody directed against an epitope located near the C-terminal end of human GDNF. Anti-GDNF antibody (#AG1175) can be used in Western blot, immunocytochemical, and immunohistochemical applications and recognizes GDNF from human and rat samples.


References 1. Lin, L.F. et al. (1993) Science 260, 1130. 2. Airaksinen, M.S. and Saarma, M. (2002) Nat. Rev. Neurosci. 3, 383. 3. Sariola, H. and Saarma, M. (2003) J. Cell Sci. 116, 3855. 4. Paratcha, G. et al. (2003) Cell 113, 867.

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$ 367.00
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Cat# AG1175-050
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