|Calculated MW||29355 Da|
|Homology||Rat, mouse- identical.|
|Other Names||Neurotrophin-3, NT-3, HDNF, Nerve growth factor 2, NGF-2, Neurotrophic factor, NTF3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)DTELLRQQRRYNSPR, corresponding to amino acid residues 89-103 of human NT-3 (precursor) (Accession P20783).ֲ Pro domain of the NT-3 protein.The antibody is specific for proNT-3, it does not crossreact with proNGF, proBDNF or mature NT-3.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF) neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5). All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways. Until recently, the functional role of the neurotrophins prodomains was thought to include assistance in the correct folding of the mature protein and the sorting of the neurotrophins into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions.Several studies implicate both proNGF and proBDNF in several physiological and pathological conditions. Today it remains to be established whether proNT-3 has similarly, different biological functions than the mature NT-3 neurotrophin. Abgent has now developed a new antibody directed against a highly specific epitope located in the pro domain region of human NT-3. The antibody does not cross reacts with mature NT-3, pro and mature NGF or pro and mature BDNF.
References 1. Hempstead, B. (2002) Curr. Opin. Neurobiol. 12, 260. 2. Blum, R. and Konnerth, A. (2005) Physiology 20, 70.
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