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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   proNT-3 Antibody   

proNT-3 Antibody

Affinity purified polyclonal antibody

  • WB - proNT-3 Antibody AG1176-050
    Western blot analysis using Anti-proNT-3 antibody (#AG1176) (1:600):
    1. hNT-3 (#N-260), (200 ng).
    2. hNT-4 (#N-270), (200 ng).
    3. mNGF 2.5S (Grade II) (#N-100), (200 ng).
    4. hß-NGF (#N-245), (200 ng).
    5. hBDNF (#B-250), (200 ng).
    6. proNGF (WT-mouse) (#N-250), (200 ng).
    7. proBDNF (WT-mouse) (#B-240), (200 ng).
    8. recombinant proNT-3 10 ng.
  • WB - proNT-3 Antibody AG1176-050
    Western blot analysis of mouse recombinant proNT-3: 1, 5. 10 ng proNT-3.
    2, 6. 50 ng proNT-3.
    3, 7. 100 ng proNT-3.
    4, 8. 200 ng proNT-3.
    Lanes 1-4 Anti-proNT-3 antibody (#AG1176), (1:300).
    Lanes 5-8 Anti-proNT-3 antibody, preincubated with the control peptide antigen.
  • IHC - proNT-3 Antibody AG1176-050
    Expression of proNT-3 in rat brain Immunohistochemical staining of rat Islands of Calleja (IOC) using Anti-proNT-3 antibody (#AG1176). A. proNT-3 staining (green) overlaps neuronal nitric oxide synthase (nNOS red) while a cluster of cells covers a part of the island (DAPI blue counterstain) but not a cell-poor area (star). B. The distribution of proNT-3 is shown alone. Note that staining is more intense in the cell poor area demarcated by white line. C. The distribution of neuronal NOS is most intense in the cell-poor region.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20783
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 29355 Da
Homology Rat, mouse- identical.
Additional Information
Gene ID 4908
Other Names Neurotrophin-3, NT-3, HDNF, Nerve growth factor 2, NGF-2, Neurotrophic factor, NTF3
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)DTELLRQQRRYNSPR, corresponding to amino acid residues 89-103 of human NT-3 (precursor) (Accession P20783).ֲ Pro domain of the NT-3 protein.The antibody is specific for proNT-3, it does not crossreact with proNGF, proBDNF or mature NT-3.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
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Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF) neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).   All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.   Until recently, the functional role of the neurotrophins prodomains was thought to include assistance in the correct folding of the mature protein and the sorting of the neurotrophins into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions.Several studies implicate both proNGF and proBDNF in several physiological and pathological conditions.   Today it remains to be established whether proNT-3 has similarly, different biological functions than the mature NT-3 neurotrophin. Abgent has now developed a new antibody directed against a highly specific epitope located in the pro domain region of human NT-3. The antibody does not cross reacts with mature NT-3, pro and mature NGF or pro and mature BDNF.


References 1. Hempstead, B. (2002) Curr. Opin. Neurobiol. 12, 260. 2. Blum, R. and Konnerth, A. (2005) Physiology 20, 70.

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$ 475.00
$ 575.00
Cat# AG1176-050
(40 western blots)
Availability: 2-3 weeks
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