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BDNF Antibody

Affinity purified polyclonal antibody

  • WB - BDNF Antibody AG1178-025
    Western blot analysis with Anti-BDNF antibody (#AG1178):
    1, 5. hBDNF (#B-250).
    2, 6. proBDNF (WT-mouse) (#B-240).
    3, 7. hß-NGF (#N-245).
    4, 8. hNT-3 (#N-260).
    Lanes 1-4 Anti-BDNF antibody (1:200).
    Lanes 5-8 Anti-BDNF antibody, preincubated with the control peptide antigen.
    Note that the antibody recognizes both BDNF and proBDNF but fails to recognize the closely related NGF and NT-3 neurotrophins.
  • IHC - BDNF Antibody AG1178-025
    Expression of BDNF in mouse cerebellum Immunohistochemical staining of mouse cerebellum with Anti-BDNF antibody (#AG1178). A. BDNF (red) appears in Purkinje cells (upward pointing arrows) and is distributed diffusely in the molecular layer (Mol) including in astrocytic fibers (downward pointing arrows). B. staining of astrocytic fibers with glial fibrillary acidic protein (green) in the same section demonstrates the distribution of BDNF to neuronal as well as to astrocytic cellular components. C. Confocal merge of BDNF and GFAP.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P23560
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 27818 Da
Homology Mouse, rat and many other species -identical.
Additional Information
Gene ID 627
Other Names Brain-derived neurotrophic factor, BDNF, Abrineurin, BDNF
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)VLEKVPVSKQLK, corresponding to amino acid residues 166-178 of human BDNF (precursor) (Accession P23560 ).The antibody is specific for BDNF; it does not crossreact with NGF, NT-3 or NT-4.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.72 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors which includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).   All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.   BDNF conveys its activity by binding to two classes of receptors, a member of the Trk receptor tyrosine kinase family (TrkB) and the pan-neurotrophin receptor p75NTR. Binding of BDNF to the TrkB receptor triggers ligand-induced dimerization and autophosphorylation of tyrosine residues. This activates various signaling cascades such the MAPK, PI3K and PLCγ pathways that are involved in cell growth, survival and differentiation of neurons in the central and peripheral nervous system.  Interestingly, recent evidence suggests that BDNF may influence target cell function via ion channel modulation. Ion channel activity in the target cells can be modulated by a TrkB-mediated mechanism that has not yet been determined. BDNF is able to block both Kv1.3 and AMPA-subtype glutamate ion channel currents in sensory neurons, while it can induce activation of the TRPC3 cation channel in neurons and of the Nav1.9 Na+ channel in hippocampal neurons. These newly recognized BDNF actions underlie its “rapid” neuronal functions that include changes in neuronal  excitability, plasticity and synaptic transmission.   Due to the high degree of homology between the different neurotrophins it has been difficult to produce truly specific antibodies against any of them. We have now produced a highly specific antibody against BDNF which recognizes exclusively BDNF.


References 1. Chao, M.V. (2003) Nature Rev. Neurosci. 4, 299. 2. Blum, R. and Konnerth, A. (2005) Physiology 20, 70. 3. Kalb, R. (2005) Trends Neurosci. 28, 5. 4. Lu, B. et al. (2005) Nature Rev. Neurosci. 6, 603.

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$ 375.00
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$ 575.00
Cat# AG1178-025
(40 western blots)
Availability: 2-3 weeks
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