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Sortilin Antibody

Affinity purified polyclonal antibody

  • WB - Sortilin Antibody AG1179-025
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate: 1, 2. Anti-Sortilin (extracellular) antibody (#AG1179), (1:400).
    3, 4. Anti-Sortilin (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Sortilin Antibody AG1179-025
    Expression of Sortilin in rat neocortex Immunohistochemical staining of rat neocortex with Anti-Sortilin (extracellular) antibody (#AG1179). A. Sortilin appears in cortical neurons (red). B. Staining of interneurons with mouse anti-parvalbumin (PV, green). C. Confocal merge of Sortilin and PV demonstrates separate localization in neocortex.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99523
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 92068 Da
Homology Mouse, rat - identical.
Additional Information
Gene ID 6272
Other Names Sortilin, 100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3, SORT1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide DKDTTRRIHVSTDQD(C) corresponding to amino acid residues 320-335 of human Sortilin (Accession Q99523). Extracellular domain.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.4 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Sortilin is a member of the Vps10p family named for a yeast gene that is involved in trafficking between the trans-golgi network and the vacuole. In mammals sortilin has been shown to play an important role in golgi to endosome and golgi to lysosome trafficking. Sortilin is also known as neurotensin receptor 3 (NTS3), one of the receptors for the peptide neurotransmitter neurotensin that exerts several biological functions ranging from the regulation of dopamine transmission and pain in the central nervous system to its functioning as a local hormone affecting the gastrointestinal tract. In addition, sortilin has been lately identified as a co-receptor of p75NTR for the binding of the proneurotrophins proNGF and proBDNF. Moreover, Sortilin expression was found to be essential for proNGF and proBDNF-induced neuronal cell death.   Sortilin is a type I membrane protein with a large extracellular domain that includes 8 bacterial neuraminidase repeats (BNRs), a single transmembrane region and a short cytoplasmic tail. The protein is expressed in several tissues, most notably in brain, spinal chord, skeletal muscle and adipocytes.   Paradoxically for its role as either a neurotensin receptor or a p75NTR co-receptor for proneurotrophins, about 90% of sortilin is retained in intracellular compartments with only about 10% reaching the cell surface. It has been speculated, that changes in the cellular environment may increase cell surface expression of sortilin.   We have now developed a new anti-Sortilin antibody directed at the extracellular region of the human sortilin protein. The antibody will recognize human rat and mouse samples.


References 1. Petersen, C.M. et al.  (1997) J. Biol. Chem. 272, 3599. 2. Mazella, J. et al. (1998) J. Biol. Chem. 273, 26273. 3. Nykjaer, A. et al. (2004) Nature 427, 843. 4. Teng, H.K. et al. (2005) J. Neurosci. 25, 5455.

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Cat# AG1179-025
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