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Nogo Receptor Antibody

Affinity purified polyclonal antibody

  • WB - Nogo Receptor Antibody AG1180-025
    Western blot analysis of rat brain lysate:
    1. Anti-Nogo Receptor (extracellular) antibody (#AG1180), (1:200).
    2. Anti-Nogo Receptor (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Nogo Receptor Antibody AG1180-025
    Expression of Nogo receptor in rat cerebellum Immunohistochemical staining of rat cerebellum using Anti-Nogo Receptor (extracellular) antibody (#AG1180).A. Nogo receptor (red) appears in Purkinje cells (arrows). B. Staining of Purkinje nerve cells with mouse anti-calbindin D28K (a calcium binding protein, green). C. Confocal merge of Nogo receptor and calbindin D28K demonstrates some co-localization of these proteins.
  • ICC - Nogo Receptor Antibody AG1180-025
    Expression of Nogo receptor in rat cerebellar granule Immunocytochemical staining of live cultured rat cerebellar granule. A. Cells were stained with Anti-Nogo Receptor (extracellular) antibody (#AG1180) followed by goat-anti-rabbit AlexaFluor-555 secondary antibody (red). Nuclei were visualized with the cell permeable dye Hoechst 33342 (blue). B. Live view of the same field as in A.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BZR6
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 50708 Da
Homology Mouse, rat - 15/16 amino acid residues identical.
Additional Information
Gene ID 65078
Other Names Reticulon-4 receptor, Nogo receptor, NgR, Nogo-66 receptor, RTN4R, NOGOR
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)GDSPPGNGSGPRHIND corresponding to amino acid residues 366-381 of the human Nogo receptor (Accession Q9BZR6). Extracellular.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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The Nogo receptor is a leucine-rich repeat (LRR) containing protein with a glycosylphosphatidylinositol (GPI) anchored C-terminus. The receptor was identified on the basis of its ability to bind with high affinity to Nogo-A a member of the reticulon family expressed in the cell membrane of oligodendrocytes.1   Nogo-A attracted attention when it was demonstrated that it is a myelin protein capable of inhibiting axonal growth following nerve injury.2,3  Other myelin proteins also identified as axonal growth inhibitors are the myelin-associated glycoprotein (MAG) a sialic-dependent immunoglobulin-like family member lectin (SIGLEC) and the oligodendrocyte myelin glycoprotein (OmgP) which is a GPI-anchored membrane protein.  Remarkably, despite their structural diversity, all three axonal growth inhibitors bind to the Nogo receptor with high affinity.4,5   Since the Nogo receptor is a GPI-anchored protein it was expected that it would require another protein component to transduce the Nogo-A binding information into the responding neurons interior. Indeed, it was shown that the neurotrophin receptor p75 (p75NTR) interacts with Nogo receptor and a third protein termed LINGO-1 to mediate axonal growth inhibition signaling.6,7   We are pleased to offer a new antibody directed against a highly conserved epitope situated in the linker region between the LRRs and the GPI anchor. The antibody will work with human, mouse and rat samples and has been tested in western blot, immunocytochemistry and immunohistochemistry techniques.


References 1. Fournier, A.E. et al. (2001) Nature 409, 341. 2. Prinjha, R. et al. (2000) Nature 403, 383. 3. GrandPré, T. et al. (2000) Nature 403, 439. 4. Domeniconi, M. et al. (2002) Neuron 35, 283. 5. Wang, K.C. et al. (2002) Nature 417, 941. 6. Wang, K.C. et al. (2002) Nature 420, 74. 7. Mi, S. et al. (2004) Nat. Neurosci. 7, 221.

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$ 475.00
Cat# AG1180-025
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