|Reactivity||Human, Mouse, Rat|
|Calculated MW||27010 Da|
|Homology||Mouse - identical; human - 16/21 amino acidresidues identical.|
|Other Names||Beta-nerve growth factor, Beta-NGF, Ngf, Ngfb|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)SPRVLFSTQPPPTSSDTLDLD, corresponding to amino acid residues 84-104 of rat NGF (precursor) (Accession number P25427). Pro domain of the NGF protein.The antibody is specific for proNGF, it does not crossreact with proBDNF, proNT-3 or mature NGF.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Neurotrophins are synthesized as pro-forms that can be cleaved either intracellularly to release mature, secreted ligands, or extracellularly by various proteases such as plasmin, furin, PC1/3, PC7, and PACE 4.1,2,5 The immature precursor has a prodomain of 103 amino acids, which was thought to have a role in the folding and sorting of the mature NGF into the various secretion pathways. It was recently reported that proNGF, binds p75NTR receptor preferentially over TrkA, and this selective binding of proNGF to p75NTR leads to apoptotic death of cells that express both TrkA and p75NTR. However, mature NGF binds and activates both receptors, with resulting promotion of cell survival due to the TrkA-mediated survival signal overriding p75NTR -mediated apoptotic signal.3,4 Since pro- and mature neurotrophins seem to elicit opposite functional effects, by differential interactions with Trks and p75NTR receptors, extracellular cleavage represents a new way to control the synaptic functions of neurotrophins. It was demonstrated that proNGF from injured spinal cord extracts, is active and induce apoptosis among oligodendrocytes, and apoptosis can be blocked by a proNGF-specific antibody.6 Finally, proNGF was demonstrated as the predominant form in mouse, rat, and human brain tissue, thyroid, hippocampus, thus suggesting a role for proNGF in vivo.5,7,8
References 1. Lessman V. et al. (2003) Progress in Neurobiology 69, 341. 2. Bibel, M. and Barde, Y.A. et al. (2000) Genes and Development 14, 2929. 3. Lee, R et al. (2001) Science 294, 1945. 4. Ibanez, C. F.et al. (2002) Trends in Neurosci. 25, 284. 5. Hassan, W. et al. (2003) J. Neurobiol. 57, 38. 6. Beattie, M. S. et al. (2002) J. Neuron. 36, 375. 7. Fahnestock, M. et al. (2001) J. Mol Cell Neurosci. 18, 210. 8. Lu, B. et al. (2003) Neuron. 39, 735.
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