|Calculated MW||22427 Da|
|Homology||Rat - identical.|
|Other Names||Neurotrophin-4, NT-4, Neurotrophin-5, NT-5, Neutrophic factor 4, NTF4, NTF5|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide LGEVPAAGGSPL(C), corresponding to amino acid residues 43-52 of mature human NT-4ֲ (123-132 of the precursor NT-4)ֲ (Accession P34130).|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.3 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||0.5 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and the PNS.1 Neurotrophin-4 (NT-4) is expressed in neurons of the superior cervical, stellate and celiac ganglion,2 T-cells3 and is synthesized by keratinocytes.4 The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.5 The rat and human forms of NT-4 are 96% homologous. NT-4 has been shown to promote dendritic outgrowth and calcium currents in cultured mesencephalic dopamine neurons,6 to promote growth and remodeling of adult motor neuron innervation,7 to be anterograde survival factors for postsynaptic cells8 and to protect against apoptotic neuronal death.9 The biological effects of NT-4 are mediated by two receptors: TrkB, which is specific for NT-4 and BDNF, and p75 which binds all the neurotrophins.10
References 1. Roux, P. et al. (2002) Prog. Neurobiol. 67, 203. 2. Roosen, A. et al. (2001) J. Neurosci. 21, 3073. 3. Moalem, G. et al. (2002) J. Autoimmun. 15, 331. 4. Marconi, A. et al. (2003) J. Invest. Dermatol. 121, 1515. 5. McDonald, N.Q. et al. (1991) Nature 354, 411. 6. DeFazio, R.A. et al. (2000) Neuroscience, 99, 297. 7. Belluardo, N. et al. (2001) Mol. Cell Neurosci. 18, 56. 8. Spalding, K.L. et al. (2002) Mol. Cell Neurosci. 19, 485. 9. Lobner, D. and Ali, C. (2002) Brain Res. 954, 42. 10. Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35.
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