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NT4 Antibody

Affinity purified polyclonal antibody

  • WB - NT4 Antibody AG1183-050
    Western blot analysis of hNT-4 (#N-270), (10 ng): 1. Anti-NT-4 antibody (#AG1183), (1:200).
    2. Anti-NT-4, preincubated with the control peptide antigen.
  • IHC - NT4 Antibody AG1183-050
    Expression of NT-4 in mouse spinal cord Immunohistochemical staining of NT-4 using Anti-NT-4 antibody (#AG1183) in mouse spinal cord. The spinal ventral horn (VH) was very lightly stained. However, in the perimeter of the white matter (WM) there were NT-4 immunoreactive cells with a small soma (triangles) and some had a stained process (arrows). 
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P34130
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 22427 Da
Homology Rat - identical.
Additional Information
Gene ID 4909
Other Names Neurotrophin-4, NT-4, Neurotrophin-5, NT-5, Neutrophic factor 4, NTF4, NTF5
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide LGEVPAAGGSPL(C), corresponding to amino acid residues 43-52 of mature human NT-4ֲ (123-132 of the precursor NT-4)ֲ (Accession P34130).
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.3 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 0.5 µg peptide per 1 µg antibody.
Research Areas
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The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and the PNS.1 Neurotrophin-4 (NT-4) is expressed in neurons of the superior cervical, stellate and celiac ganglion,2 T-cells3 and is synthesized by keratinocytes.4 The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.5 The rat and human forms of NT-4 are 96% homologous. NT-4 has been shown to promote dendritic outgrowth and calcium currents in cultured mesencephalic dopamine neurons,6 to promote growth and remodeling of adult motor neuron innervation,7 to be anterograde survival factors for postsynaptic cells8 and to protect against apoptotic neuronal death.9   The biological effects of NT-4 are mediated by two receptors: TrkB, which is specific for NT-4 and BDNF, and p75 which binds all the neurotrophins.10


References 1. Roux, P. et al. (2002) Prog. Neurobiol. 67, 203. 2. Roosen, A. et al. (2001) J. Neurosci. 21, 3073. 3. Moalem, G. et al. (2002) J. Autoimmun. 15, 331. 4. Marconi, A. et al. (2003) J. Invest. Dermatol. 121, 1515. 5. McDonald, N.Q. et al. (1991) Nature 354, 411. 6. DeFazio, R.A. et al. (2000) Neuroscience, 99, 297. 7. Belluardo, N. et al. (2001) Mol. Cell Neurosci. 18, 56. 8. Spalding, K.L. et al. (2002) Mol. Cell Neurosci. 19, 485. 9. Lobner, D. and Ali, C. (2002) Brain Res. 954, 42. 10. Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35.

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$ 367.00
$ 475.00
Cat# AG1183-050
Availability: 2-3 weeks
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