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NT3 Antibody

Affinity purified polyclonal antibody

  • WB - NT3 Antibody AG1184-025
    Western blot analysis using Anti-NT-3 antibody (1:1000) (1-4) or anti-NT-3 antibody, preincubated with the control antigen peptide (5): 1. hNT-3 (#N-260), 10 ng
    2. hß-NGF (#N-245), 100 ng
    3. hNT-4 (#N-260), 100 ng
    4. hNT-3 (#N-260), 10 ng
    5. Control peptide
  • IHC - NT3 Antibody AG1184-025
    Expression of NT-3 in mouse spinal cord Immunohistochemical staining of mouse spinal cord using Anti-NT-3 antibody (#AG1184). The spinal ventral horn was stained diffusely with some extensions (arrows) into the white matter (WM).  In the WM there were NT-3 immunoreactive cells that were probably glia (triangles).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20783
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 29355 Da
Homology Rat, mouse, chicken, Xenopus - identical; cat - 9/10 amino acidresidues identical.
Additional Information
Gene ID 4908
Other Names Neurotrophin-3, NT-3, HDNF, Nerve growth factor 2, NGF-2, Neurotrophic factor, NTF3
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide GEIKTGNSPV(C),ֲ corresponding to amino acid residues 39-48 of mature human NT-3ֲ (residues 177-186 of the NT-3 precursor) (Accession P20783).
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Application Details Immunohistochemistry (IH): Rat hippocampus (1:300) (see Ramon Languren, L.E. and Escobar, M.L. (2013) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.3 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 0.5 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
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The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and the PNS.1 The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.2 The rat and human forms of Neurotrophin-3 (NT-3) are 96% homologous.   NT-3 has been shown to strengthen synaptic connections to motoneurons in the neonatal rat,3 to serve as an anti-inflammatory factor, to suppress microglial activation,4 to play a critical role in regulating T helper 1/T helper 2 cell balance5 and to modify potassium currents in isolated inner hair cells from guinea pig cochlea.6 The biological effects of NT-3 are mediated by two receptors: TrkC, which is specific for NT-3, and p75NTR, which binds all the neurotrophins.7


References 1. Roux, P.P. and Barker, P.A. (2002) Prog. Neurobiol. 67, 203. 2. McDonald N.Q. et al. (1991) Nature 354, 411. 3. Arvanian, V.L. et al. (2003) J. Neurosci. 23, 8706. 4. Tzeng, S.F. and Huang, H.Y. (2003) J. Cell Biochem. 90, 227. 5. Sekimoto, M. et al. (2003) Immunol. Lett. 88, 221. 6. Kimitsuki, T. et al. (2003) Auris. Nasus Larynx. 30, 141. 7. Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35.

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Cat# AG1184-025
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