|Reactivity||Human, Mouse, Rat|
|Calculated MW||29355 Da|
|Homology||Rat, mouse, chicken, Xenopus - identical; cat - 9/10 amino acidresidues identical.|
|Other Names||Neurotrophin-3, NT-3, HDNF, Nerve growth factor 2, NGF-2, Neurotrophic factor, NTF3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide GEIKTGNSPV(C),ֲ corresponding to amino acid residues 39-48 of mature human NT-3ֲ (residues 177-186 of the NT-3 precursor) (Accession P20783).|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Immunohistochemistry (IH): Rat hippocampus (1:300) (see Ramon Languren, L.E. and Escobar, M.L. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.3 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||0.5 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and the PNS.1 The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.2 The rat and human forms of Neurotrophin-3 (NT-3) are 96% homologous. NT-3 has been shown to strengthen synaptic connections to motoneurons in the neonatal rat,3 to serve as an anti-inflammatory factor, to suppress microglial activation,4 to play a critical role in regulating T helper 1/T helper 2 cell balance5 and to modify potassium currents in isolated inner hair cells from guinea pig cochlea.6 The biological effects of NT-3 are mediated by two receptors: TrkC, which is specific for NT-3, and p75NTR, which binds all the neurotrophins.7
References 1. Roux, P.P. and Barker, P.A. (2002) Prog. Neurobiol. 67, 203. 2. McDonald N.Q. et al. (1991) Nature 354, 411. 3. Arvanian, V.L. et al. (2003) J. Neurosci. 23, 8706. 4. Tzeng, S.F. and Huang, H.Y. (2003) J. Cell Biochem. 90, 227. 5. Sekimoto, M. et al. (2003) Immunol. Lett. 88, 221. 6. Kimitsuki, T. et al. (2003) Auris. Nasus Larynx. 30, 141. 7. Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35.
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