|Calculated MW||94294 Da|
|Homology||Mouse-identical; human - 13/14 amino acid residues identical.|
|Other Names||Neuroligin-1, Neuroligin I, Nlgn1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)KVPSTDITLRPTRK, corresponding to amino acid residues 648-661 of rat Neuroligin 1 (Accession Q62765). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The NLGN1 gene encodes a member of a family of neuronal cell surface proteins. Neuroligins act as ligands for β-Neurexins, cell adhesion proteins located presynaptically. Neuroligin and β-neurexin "shake hands," resulting in the connection between the two neurons and the production of a synapse. Neuroligin 1 may be involved in the formation and remodeling of central nervous system synapses1. Proteins of this family may also mediate signaling by recruiting and stabilizing key synaptic components. This is done by interacting with other postsynaptic proteins to localize neurotransmitter receptors and channels in the postsynaptic density as the cell matures1. Mutations in the Neuroligin genes are implicated in autism and other cognitive disorders2. Neuroligins have been found to play a role in angiogenesis, specifically in human pheripheral tissues3. Neuroligins bind with the help of Ca2+ to the α-neurexin LNS (laminin, neurexin and sex hormone-binding globulin-like folding units) domains and to the β-neurexin LNS domain which then establishes a heterophilic trans-synaptic recognition code4. This binding results in a heterotetramer made up of a Neuroligin-1 dimer and two Neurexin-1 β monomers5.
References 1. Scheiffele, P. et al. (2000) Cell 101, 657. 2. Sudhof, T.C. et al. (2008) Nature 455, 903-911. 3. Bottos, A. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106, 20782. 4. Fabrichny, I.P. et al. (2007) Neuron 56, 979. 5. Arac, D. et al. (2007) Neuron 56, 992.
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