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Neuropeptide Y5 Receptor Antibody

Affinity purified polyclonal antibody

  • WB - Neuropeptide Y5 Receptor Antibody AG1186-050
    Western blot analysis of rat brain membrane:

    1. Anti-Neuropeptide Y5 Receptor antibody (#AG1186), (1:200).
    2. Anti-Neuropeptide Y5 Receptor antibody, preincubated with the control peptide antigen.

  • IHC - Neuropeptide Y5 Receptor Antibody AG1186-050
    Expression of Neuropeptide Y5 Receptor in rat striatum Immunohistochemical staining of rat striatum using Anti-Neuropeptide Y5 Receptor antibody (#AG1186). A. NPY5R (red) appears in the striatal matrix (asterisk) and in nerve cells in the matrix (vertical arrows). B. Parvalbumin (green) appears in the striatal matrix. C. Merge of NPY5R and parvalbumin demonstrates co-localization in a sub-set of cells (horizontal arrows). DAPI is used as the counterstain (blue).
  • ICC - Neuropeptide Y5 Receptor Antibody AG1186-050
    Expression of NPY5R in rat DRG Immunocytochemical staining of a primary culture of rat dorsal root ganglion (DRG) neurons. A, D. A paraformaldehyde-fixed and permeabilized DRG primary culture was stained with Anti-Neuropeptide Y5 Receptor antibody (#AG1186), (1:100) followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B. F. Merged images of panels D and E.
    A-C: x20
    E-F: x100
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q63634
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 50408 Da
Homology Mouse, bovine, canis- identical; human- 15/16 amino acid residues identical.
Additional Information
Gene ID 25340
Other Names Neuropeptide Y receptor type 5, NPY5-R, NPY-Y5 receptor, NPYY5-R, Y5 receptor, Npy5r, Npyr5
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide SFIRKHRRRYSKKTAC corresponding to amino acid residues 291-306 of rat NPY5R (Accession Q63634 ). 3rd intracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and mass-spectrography.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.7 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 2 µg peptide per 1 µg antibody.
Research Areas
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Neuropeptide Y (NPY) is a 36 amino acid peptide neurotransmitter present in the central and peripheral nervous systems.1 NPY is involved in the regulation of a broad range of functions such as eating behavior, hypertension, and reproduction.2 NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R).2 Four of these (Y1, Y2, Y4, and Y5) are expressed in humans.2 The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus. Activation of these receptors by NPY affects [Ca2+]i, adenylate cyclase signalling, and a number of ion channel functions.3 Based on their high frequency and density in steroid hormone-producing tumors, the NPYRs might be of potential use in tumor management.2   NPY5R as well as NPY1R and NPY2R are expressed in the hypothalamus, in the brain stem, and in peripheral tissues, such as blood vessels, lung, kidney, adrenal glands, stomach, colon, heart, pancreas, and intestine. NPY5R has been postulated as the mediator of the feeding response to NPY and related peptides (125 in Kamiji, M. M). Its localization in the brain is consistent with this hypothesis.4   Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat Neuropeptide Y5 receptor (NPY5R). Anti-Neuropeptide Y5 Receptor antibody (#AG1186) can be used for Western blot analysis, immunohistochemical and immunocytochemical applications. It has been designed to recognize NPY5R from rat samples, and based on the sequence homology is expected to recognize NPY5R from human and mouse samples as well.


References 1. Stanic, D. et al. (2006) J. Comp. Neurol. 499, 357. 2. Körner, M. et al. (2004) Clin. Cancer Res. 10, 8426. 3. Sun, L. and Miller, R.J. (1999) J. Neurophysiol. 81, 1391. 4. Kamiji, M. M. and. Inui, A (2007) Endocrine Reviews 28, 664.

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$ 367.00
$ 475.00
Cat# AG1186-050
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