|Calculated MW||50408 Da|
|Homology||Mouse, bovine, canis- identical; human- 15/16 amino acid residues identical.|
|Other Names||Neuropeptide Y receptor type 5, NPY5-R, NPY-Y5 receptor, NPYY5-R, Y5 receptor, Npy5r, Npyr5|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide SFIRKHRRRYSKKTAC corresponding to amino acid residues 291-306 of rat NPY5R (Accession Q63634 ). 3rd intracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.7 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||2 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Neuropeptide Y (NPY) is a 36 amino acid peptide neurotransmitter present in the central and peripheral nervous systems.1 NPY is involved in the regulation of a broad range of functions such as eating behavior, hypertension, and reproduction.2 NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R).2 Four of these (Y1, Y2, Y4, and Y5) are expressed in humans.2 The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus. Activation of these receptors by NPY affects [Ca2+]i, adenylate cyclase signalling, and a number of ion channel functions.3 Based on their high frequency and density in steroid hormone-producing tumors, the NPYRs might be of potential use in tumor management.2 NPY5R as well as NPY1R and NPY2R are expressed in the hypothalamus, in the brain stem, and in peripheral tissues, such as blood vessels, lung, kidney, adrenal glands, stomach, colon, heart, pancreas, and intestine. NPY5R has been postulated as the mediator of the feeding response to NPY and related peptides (125 in Kamiji, M. M). Its localization in the brain is consistent with this hypothesis.4 Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat Neuropeptide Y5 receptor (NPY5R). Anti-Neuropeptide Y5 Receptor antibody (#AG1186) can be used for Western blot analysis, immunohistochemical and immunocytochemical applications. It has been designed to recognize NPY5R from rat samples, and based on the sequence homology is expected to recognize NPY5R from human and mouse samples as well.
References 1. Stanic, D. et al. (2006) J. Comp. Neurol. 499, 357. 2. Körner, M. et al. (2004) Clin. Cancer Res. 10, 8426. 3. Sun, L. and Miller, R.J. (1999) J. Neurophysiol. 81, 1391. 4. Kamiji, M. M. and. Inui, A (2007) Endocrine Reviews 28, 664.
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