|Calculated MW||42568 Da|
|Homology||Mouse- identical; human- 13/15 amino acid residues identical.|
|Other Names||Neuropeptide Y receptor type 4, NPY4-R, Pancreatic polypeptide receptor 1, PP1, Npy4r, Ppyr1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide NINFKKDIKALVLTC corresponding to amino acid residues 326-340 of rat NPY4R (Accession Q63447). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.6 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Neuropeptide Y (NPY) is a 36 amino acid peptide neurotransmitter in the central (CNS) and peripheral nervous systems.1 It belongs to the NPY family, along with peptide YY (PYY) and pancreatic polypeptide (PP). NPY is involved in the regulation of a broad range of homeostatic functions such as eating behavior, hypertension, and reproduction.2 NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R).2 Four of these (Y1, Y2, Y4, and Y5) are expressed in humans.2 The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus. Activation of these receptors by NPY produces effects on [Ca2+]i, adenylate cyclase, and a number of ion channels.3 Based on their high frequency and density in steroid hormone-producing tumors, the NPYRs might be of potential use in tumor management.2 NPY4R preferentially binds PP, while NPY1R, NPY2R, and NPY5R bind both NPY and PP. NPY1R, NPY2R, and NPY5R share a low degree of identity (27–31% overall). NPY4R is more similar to NPY1R, displaying an overall identity of 43–53%.4 Human NPY4R (also named pancreatic polypeptide receptor 1) mRNA is highly expressed in the small and large intestines and the prostate. Tissue distribution studies in human and mouse suggest potential roles for NPY4R in the gastrointestinal tract, heart, and prostate, as well as in neural and endocrine signaling. Only low levels of NPY4R expression in various areas of brain were displayed.5 Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat Neuropeptide Y4 receptor (NPY4R). The Anti-Neuropeptide Y4 Receptor antibody (#AG1187) can be used for Western blot analysis and immunocytochemical applications and should recognize NPY4R from human, rat, and mouse samples.
References 1. Stanic, D. et al. (2006) J. Comp. Neurol. 499, 357. 2. Körner, M. et al. (2004) Clin. Cancer Res. 10, 8426. 3. Sun, L. and Miller, R.J. (1999) J. Neurophysiol. 81, 1391. 4. Larhammar, D. et al. (2001) Peptides 22, 295. 5. Voisin, T. et al. (2000) J. Pharmacol. Exp. Ther. 292, 638.
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