|Calculated MW||42628 Da|
|Homology||Rat - identical; human - 18/19 amino acid residues identical.|
|Other Names||Neuropeptide Y receptor type 2, NPY2-R, NPY-Y2 receptor, Y2 receptor, Npy2r|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CEQRLDAIHSEVSMTFKAK, corresponding to amino acid residues 346-364 of mouse NPY2R (Accession P97295). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||1 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Neuropeptide Y (NPY) is a 36-amino acid peptide neurotransmitter in the central (CNS) and peripheral nervous systems.1 It belongs to the NPY family, together with peptide YY (PYY) and pancreatic polypeptide (PP). NPY is involved in regulation of a broad range of homeostatic functions such as eating behavior, hypertension, and reproduction.2 NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R).2 Four of these, Y1, Y2, Y4, and Y5, are expressed in humans.2 The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus. Activation of these receptors by NPY produces effects on [Ca2+]i, adenylate cyclase, and a number of ion channels.3 Based on the high frequency and density of NPY receptors in steroid hormone-producing tumors, the NPY receptors might be of potential use in tumor management.2 NPY2R is primarily expressed in neurons as a presynaptic receptor. It is also strongly expressed in epithelia of the visceral tissues, including colon and kidney.4 NPY2R is involved in inhibition of neurotransmitter release mediated by NPY.5 Release of glutamate, the principal neuroexcitatory transmitter, is inhibited via the NPY2R receptor.6 Involvement of NPY2R in feeding behavior was also reported. PYY(3–36), an NPY2R agonist, acts to inhibit feeding via NPY2R.7
References 1. Stanic, D. et al. (2006) J. Comp. Neurol. 499, 357. 2. Körner, M. et al. (2004) Clin. Cancer Res. 10, 8426 3. Sun, L. and Miller, R.J. (1999) J. Neurophysiol. 81, 1391. 4. Bournat, J.C. and Allen, J.M. (2001) Brain Res. Mol. Brain Res. 90, 149. 5. Wheway, J. et al. (2005) J. Exp. Med. 202, 1527. 6. Silva, A.P. et al. (2006) FASEB J. 21, 671. 7. Batterham, R.L. et al. (2003) N. Engl. J. Med. 349, 941.
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