|Application ||WB, IHC, ICC|
|Calculated MW||44392 Da|
|Homology||Rat, mouse - 14/16 amino acid residues identical.|
|Other Names||Neuropeptide Y receptor type 1, NPY1-R, NPY1R, NPYR, NPYY1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)RLKRRNNMMDKMRDNK, corresponding to amino acid residues 237-252 of human NPY1R (Accession P25929). 3rd intracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Neuropeptide Y (NPY) is a 36-amino acid peptide neurotransmitter in the central (CNS) and peripheral nervous systems.1 It belongs to the NPY family, together with the peptide YY (PYY) and the pancreatic polypeptide (PP). NPY is involved in regulation of a broad range of homeostatic functions such as eating behavior, hypertension, and reproduction.2 NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R) and Y6 (NPY6R).2 Four of these, Y1, Y2, Y4, and Y5, are expressed in humans.2 The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus. Activation of these receptors by NPY produces effects on [Ca2+]i, adenylate cyclase, and a number of ion channels.3 Based on the high frequency and density of NPY receptors in steroid hormone-producing tumors, the NPY receptors might be of potential use in tumor management.2 NPY1R is considered to be a postsynaptic receptor. In the CNS, NPY1Rs are concentrated in the cortical areas of the brain, cerebral cortex, olfactory nucleus, hippocampus, hypothalamic nuclei, and thalamic nuclei.4 In addition, this receptor has a bimodal role in the immune system, serving as a strong negative regulator on T cells as well as a key activator of antigen-presenting cell function.5 Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of the human neuopeptide Y1 receptor (NPY1R). The Anti-Neuropeptide Y1 Receptor antibody (#AG1189) can be used for western blot analysis and immunocytochemical staining applications, and should recognize NPY1R from human, rat, and mouse samples.
1. Stanic, D. et al. (2006) J. Comp. Neurol. 499, 357.
2. Körner, M. et al. (2004) Clin. Can. Res. 10, 8426.
3. Sun, L. and Miller, R.J. (1999) J. Neurophysiol. 81, 1391.
4. Bournat, J.C. and Allen, J.M. (2001) Brain Res. Mol. Brain Res. 90, 149.
5. Wheway, J. et al. (2005) J. Exp. Med. 202, 1527.
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