|Calculated MW||33359 Da|
|Homology||Human - 16/17 amino acid residues identical; mouse - 15/17 amino acid residues identical.|
|Other Names||Syntaxin-2, Epimorphin, Stx2, Epim|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide RDRLPDLTACRKSDDGDN, corresponding to amino acid residues 2-19 of rat Syntaxin 2 (Accession P50279). N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.3 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Syntaxin 2 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60–70 amino acids that are arranged in heptad repeats1,2. SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2. SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion. Syntaxin 2, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located in the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2. Syntaxin 2 functions as a plasma membrane protein t-SNARE, which together with vesicular v-SNARE proteins is involved in regulated exocytosis in cells such as platelets, sperm cells and pancreatic acinar cells3-5. Intriguingly, Syntaxin 2 has been also identified as epimorphin, an extracellular membrane protein implicated in branching morphogenesis, a developmental process essential for the formation of many organs, including mammary gland, lung, kidney, and salivary gland6. Abgent is pleased to offer a highly specific antibody directed against an epitope located in the N-terminus domain of rat Syntaxin 2. Anti-Syntaxin 2 antibody (#AG1190) can be used in western blot and immunohistochemical applications, and was designed to recognize Syntaxin 2 from rat, mouse and human samples.
References 1. Jahn, R. and Scheller, R.H. (2006) Nat. Rev. Mol. Cell Biol. 7, 631. 2. Südhof, T.C. and Rothman, J.E. (2009) Science 323, 474. 3. Chen, D.et al (2000) Blood 95, 921. 4. Hutt, D.M. et al (2005) J. Biol. Chem. 280, 20197. 5. Hansen, N.J .et al (1999) J. Biol. Chem. 274, 22871. 6. Radisky, D.C. et al (2003) Trends Cell. Biol. 13, 426.
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